Human melanocyte growth supplement 2 hmgs 2

Chlorpromazine hydrochloride, phosphated-buffered saline (PBS), l-3,4-dihydroxyphenylalanine (l-DOPA), and amphotericin B were purchased from Sigma-Aldrich Inc. (USA). Neomycin sulfate was obtained from Amara (Poland). Penicillin was acquired from Polfa Tarchomin (Poland). Growth medium M-254 and human melanocyte growth supplement-2 (HMGS-2) were obtained from Cascade Biologics (UK). Trypsin/EDTA was obtained from Cytogen (Poland). Cell Proliferation Reagent WST-1 was purchased from Roche GmbH (Germany). The remaining chemicals were produced by POCH S.A. (Poland).

Norfloxacin, phosphated-buffered saline (PBS), 3,4-dihydroxy-l-phenylalanine (L-DOPA) and amphotericin B were purchased from Sigma-Aldrich Inc.(USA). moxifloxacin hydrochloride (Avelox^TM solution for i.v. use containing 400 mg of moxifloxacin in 0.8 % saline) was obtained from Bayer Healthcare Pharmaceuticals Inc. (Germany). Neomycin sulfate was obtained from Amara (Poland). Penicillin was acquired from Polfa Tarchomin (Poland). Growth medium M-254 and human melanocyte growth supplement-2 (HMGS-2) were obtained from Cascade Biologics (UK). Trypsin/EDTA was obtained from Cytogen (Poland). Cell Proliferation Reagent WST-1 was purchased from Roche GmbH (Germany). The remaining chemicals were produced by POCH S.A. (Poland).

Minocycline hydrochloride, C₂₃H₂₇N₃O₇ x HCl, SIGMAFAST Protease Inhibitor Cocktail Tablets, EDTA-Free and Phosphatase Inhibitor Cocktail 3, DCFH₂-DA (2′,7′-dichlorofluorescein diacetate), penicillin, amphotericin B solution (250 µg/mL), and phosphate-buffered saline (PBS) were obtained from Sigma-Aldrich Inc. (Taufkirchen, Germany). An M-254 growth medium and a human melanocyte growth supplement-2 (HMGS-2) were purchased from Cascade Biologics (Portland, OR, USA). Neomycin sulfate was acquired from Amara (Kraków, Poland). Trypsin/EDTA was obtained from Cytogen (Zgierz, Poland). Cell Proliferation Reagent WST-1 was acquired from Roche GmbH (Mannheim, Germany). Dulbecco’s phosphate-buffered saline (DPBS), primary anti-tyrosinase monoclonal antibody, secondary antibody conjugated with Alexa Fluor 488 as well as DAPI (4′6-diamidino-2-phenylindole, dihydrochloride) 1 mg/mL in water were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Solution 5 (400 μg/mL VitaBright-48™, 500 μg/mL propidium iodide, 1.2 μg/mL acridine orange in DMSO), as well as Via-1-Cassettes™ (acridine orange and DAPI fluorophores) and NC-Slides™ A8 were obtained from ChemoMetec (Lillerød, Denmark). The remaining reagents and chemicals were produced by POCH SA (Gliwice, Poland).