Urine and serum were collected and analyzed for mouse urine albumin excretion using an ELISA kit (Bethyl Laboratories, Montgomery, TX, USA) as described previously26 (link) and blood urea nitrogen (BUN) with a Beckman Autoanalyzer (Beckman Coulter, Fullerton, CA, USA), respectively. Urine albumin concentration was normalized to urine creatinine concentrations measured using the creatinine kit Stanbio Creatinine Procedure No. 0400 (Stanbio Laboratory, Boerne, TX, USA). Albumin excretion in normal mice is <0.025 mg/mg creatinine.
Beckman autoanalyzer
Manufactured by Beckman Coulter
Sourced in United States
The Beckman Autoanalyzer is an automated laboratory instrument designed for chemical analysis. It is capable of performing a variety of clinical laboratory tests, including but not limited to measurements of blood chemistry, enzyme activity, and drug levels. The Autoanalyzer automates sample handling, reagent addition, and data processing, allowing for efficient and consistent analysis of multiple samples.
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Lab products found in correlation
11 protocols using beckman autoanalyzer
1
Quantifying Urine Albumin Excretion in Mice
2
Post-Reperfusion Graft Evaluation Protocol
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Serum total creatine phosphokinase (CPK) was assayed using a Beckman autoanalyzer (Beckman Instruments, Fullerton, CA, USA). We evaluated the gross morphologyof the grafts with their identities masked and assigned a transplant score based on color (1: dead; 2: dark; 3: partially dark; 4: nearly healthy; 5: healthy or normal), contractility (1: dead; 2: not much; 3: moderate function; 4: pretty good; 5: excellent function or normal) and hardness (1: dead; 2:hard; 3: partially hard; 4:nearly soft; 5: soft or normal) at 6 hours post-reperfusion.
3
Serum, Urine Biomarkers Evaluation
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Using a Beckman Autoanalyzer (Beckman Coulter), the level of serum creatinine (SCr) and blood urea nitrogen (BUN) were tested. An ELISA Kit from Cosmo Bio and an ELISA Kit from Sangon Biotech were respectively used to examine the urine concentration of Kim-1 and the cultured concentration of TNF-α, IL-6, and IL-1β.
4
Quantifying Serum Antibodies and Immune Complexes
5
Metabolic Biomarkers in Rodent and Cell Models
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Serum creatine kinase (CK), CK isoenzyme-MB (CK-MB), blood urea nitrogen (BUN), serum creatinine (Scr), K+, Na+, Cl-, and glucose levels in rats or mice and extracellular K+ and glucose levels in cultured C2C12 myoblasts were measured with an autoanalyzer (Beckman Autoanalyzer; Beckman Instruments, Fullerton, CA, USA). Rats and mice serum insulin levels were determined by ELISA according to the manufacturer’s instructions (Shibayagi, Gunma, Japan). The homeostasis model assessment of insulin resistance (HOMA-IR) index was calculated according to the following formula: HOMA-IR = fasting serum insulin (mIU/L) × fasting serum glucose (mmol/L)/22.5. The quantitative insulin sensitivity check index (QUICKI) was calculated using the original formula as the inverse log sum of fasting serum insulin in mIU/L and fasting serum glucose in mg/dl. QUICKI = 1/[log(fasting serum glucose) + log(fasting serum insulin)].
6
Measuring Autoimmune Antibodies and Immune Complexes
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Blood urea nitrogen concentrations were measured with a Beckman Autoanalyzer (Beckman Coulter, Fullerton, CA). Serum anti-apoferritin IgG antibodies were measured by enzyme-linked immunosorbent assay.9 (link) Briefly, 96-well plates were coated with apoferritin. Serial dilutions of sera were plated and incubated at room temperature for 2 h, followed by HRP-conjugated goat anti-mouse IgG (Kierkegard & Perry Laboratories, Gaithersburg, MD) and OPD peroxidase substrate (Sigma-Aldrich, St. Louis, MO). The OD450 was then measured.
Circulating IC levels were measured by C1q-binding enzyme-linked immunosorbent assay, as described previously.41 (link) Briefly, 96-well plates were coated with 10 μg/ml human C1q (Sigma-Aldrich) in carbonate buffer. After blocking with 1% bovine serum albumin, sera samples were loaded in serial dilutions starting at 1/1000, followed by HRP-goat anti-mouse IgG (Sigma, 1:2000) and then TMB (Jackson ImmunoResearch Laboratories, West Grove, PA). The OD450 was then measured. Sera samples from unmanipulated wild-type mice were used as negative control.
Circulating IC levels were measured by C1q-binding enzyme-linked immunosorbent assay, as described previously.41 (link) Briefly, 96-well plates were coated with 10 μg/ml human C1q (Sigma-Aldrich) in carbonate buffer. After blocking with 1% bovine serum albumin, sera samples were loaded in serial dilutions starting at 1/1000, followed by HRP-goat anti-mouse IgG (Sigma, 1:2000) and then TMB (Jackson ImmunoResearch Laboratories, West Grove, PA). The OD450 was then measured. Sera samples from unmanipulated wild-type mice were used as negative control.
7
Metabolic Biomarker Assessment Protocol
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Serum fasting glucose, triglyceride (TG), total cholesterol (TC), high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C) were measured by the enzymatic colorimetric method (Beckman Autoanalyzer, Beckman Instruments, Palo Alto, CA, USA) using commercially appropriate kits (Noeman, Hamooda & Baalash, 2011 (link)). Serum insulin (Sunredbio, China), leptin (Sunredbio, China) and ghrelin (Sunredbio, China) analyses were performed using commercially available enzyme-linked immunosorbent assay kits. Homeostasis Model Assessment of Insulin (HOMA-IR) was calculated using the following formula: [HOMA-IR = (insulin (U/l) * glucose (mg/dl)/405] (Matthews et al., 1985 (link)).
8
Kidney Biomarker Measurement Protocol
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The levels of SCr and blood urea nitrogen (BUN) were tested using a Beckman Autoanalyzer (Beckman Coulter, Fullerton, CA, United States). An ELISA Kit from Cosmo Bio (Tokyo, Japan) and an ELISA Kit from Sangon Biotech (Shanghai, China) were, respectively, used to examine the urine concentration of kidney injury molecule 1 (KIM-1) and the cultured concentration of tumor necrosis factor-α (TNF-α), interleukin (IL)-6, IL-10, and IL-1β (Yuan et al., 2021 (link)).
9
Insulin Sensitivity Assessment Protocol
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Glucose and insulin were measured via venous blood samples collected fasted and 2 h post-challenge. Glucose samples were analysed using a glucose oxidase method on the Beckman Auto Analyzer (Beckman, High Wycombe, UK) within a laboratory at the Leicester Royal Infirmary. Plasma samples were frozen at −80 °C for insulin analysis at the end of data collection using an enzyme immunoassay (80-INSHU-E01.1, E10.1 Alpco Diagnostics 26G Keewaydin Drive, Salem, NH, USA) at a specialist laboratory by Unilever R&D, Bedfordshire, UK. For this analysis, two-hour glucose and two-hour insulin were used. The homeostasis model assessment of insulin sensitivity (HOMA-IS) and the Matsuda insulin sensitivity index (Matsuda-ISI) were both calculated to estimate insulin sensitivity [35 (link),36 (link)], as follows:
10
Lipid and Glucose Assay Protocol
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Total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) levels were determined by enzymatic colorimetric test technique. Fasting blood glucose (FPG) was determined by an automated glucose oxidation method in a Beckman autoanalyzer (Beckman Instruments, Palo Alto, CA).
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