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Dreg 56

Manufactured by BD
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Sourced in United States

The DREG-56 is a laboratory equipment designed for the separation and purification of biological samples. It utilizes a centrifugal force to create a stable pressure gradient, allowing for the efficient separation of components within the sample. The DREG-56 is a versatile tool suitable for a wide range of applications in various research and diagnostic settings.

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Lab products found in correlation

Ifn γ xmg1.2, by Thermo Fisher Scientific (2 mentions) Cd25 pc61, by Thermo Fisher Scientific (2 mentions) Cd44 im7, by BD (2 mentions) Cd3ε γ 17a2, by BioLegend (2 mentions) Cd62l mel 14, by Thermo Fisher Scientific (2 mentions) Cd126 d7715a7, by Thermo Fisher Scientific (2 mentions) Cd127 25 1271 82, by Thermo Fisher Scientific (2 mentions) βtcr h57 597, by R&D Systems (2 mentions) Gp130 125623, by R&D Systems (2 mentions) Il 4 11b11, by Thermo Fisher Scientific (2 mentions) Recombinant mouse il 21r fc chimera protein, by R&D Systems (2 mentions) Il 21 receptor antibody, by Jackson ImmunoResearch (2 mentions) Af1930, by R&D Systems (2 mentions) Uchl1, by BioLegend (2 mentions) Cd197 ccr7 g043h7, by BioLegend (2 mentions) Rpa t4, by Thermo Fisher Scientific (2 mentions) Cd4 rm4 5, by Thermo Fisher Scientific (2 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions) Facscanto 2 flow cytometer, by BD (1 mentions) Saponin buffer, by Merck Group (1 mentions)

Close spelling variants of this product

CD62L-BV605 (clone DREG-56), CD62L (DREG-56, CD62L FITC (clone DREG-56) Anti-CD62L (clone DREG-56, Anti CD62L PE (clone DREG-56), APC-anti-human CD62L (clone DREG-56,

5 protocols using dreg 56

1

Characterization of T Cell Subsets

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Mouse specific antibodies against CD3ε/γ (17A2; Biolegend), CD4 (RM4-5; eBioscience), CD25 (PC61.5; eBioscience), CD44 (IM7; BD Biosciences), CD62L (MEL-14; Life Technologies), CD126 (D7715A7; eBioscience), CD127 (25-1271-82; eBioscience),βTCR (H57-597), gp130 (125623; R&D Systems), IFNγ (XMG1.2; eBioscience), IL-4 (11B11; eBioscience), IL-17A (TC11-18H10.1; Biolegend), IL-21 (Recombinant mouse IL-21R Fc Chimera protein; R&D and IL-21 receptor antibody; Jackson Immuno Research) and PTPN2 (AF1930; R&D) were used. For detection of human antigens, we used antibodies specific to CD3 (UCHT1; BioLegend), CD4 (RPA-T4; eBioscience), CD45RA (HI100; BioLegend), CD45RO (UCHL1; BioLegend), CD62L (DREG-56; BD Biosciences), CD197 (CCR7; G043H7; BioLegend). Human and mouse cross-specific antibodies to pY STAT1 (pY701; 4a), pY-STAT3 (pY705; 4/P-STAT3), pS-STAT1 (pS727; K51-856) and pS STAT3 (pS727, 49/p-Stat3) were from BD Biosciences.
Twohig J.P., Figueras A.C., Andrews R., Wiede F., Cossins B.C., Soria A.D., Lewis M.J., Townsend M.J., Millrine D., Li J., Hill D.G., Fernandez J.U., Liu X., Szomolay B., Pepper C.J., Taylor P.R., Pitzalis C., Tiganis T., Williams N.M., Jones G.W, & Jones S.A. (2019). Activation of naïve CD4+ T cells re-tunes STAT1 signaling to deliver unique cytokine responses in memory CD4+ T cells. Nature immunology, 20(4), 458-470.
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2

Neutrophil Activation Markers Expression

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Neutrophils (1 × 105) were incubated with tumor necrosis factor (TNF-α; 10 ng/mL Peprotech, London, UK) or the isolated S100A8/A9 fraction for 2 h at 37 °C. Flow cytometry was performed to assess the expression of neutrophil activation markers. Antibodies used were CD11b-FITC (mouse IgM clone CLB-mon-gran/1, B2, Sanquin reagents) and L-selectin-APC (mouse IgG clone DREG-56, BD Pharmingen, San Diego, CA, USA). Cells were analyzed on a FACSCanto-II flow cytometer (BD Biosciences, San Jose, CA, USA). Neutrophils were gated based on their forward- and side-scatter, and 10,000 gated events per sample were collected. Data are expressed as mean fluorescence intensity (MFI) and are corrected for relevant isotype controls.
Sprenkeler E.G., Zandstra J., van Kleef N.D., Goetschalckx I., Verstegen B., Aarts C.E., Janssen H., Tool A.T., van Mierlo G., van Bruggen R., Jongerius I, & Kuijpers T.W. (2022). S100A8/A9 Is a Marker for the Release of Neutrophil Extracellular Traps and Induces Neutrophil Activation. Cells, 11(2), 236.
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3

Flow Cytometric Analysis of T-Cell Markers

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Cultured cells were washed twice in PBS containing 1 % BSA and stained with monoclonal antibodies specific for cell-surface markers. Antibodies to CD4 (RPA-T4), CD8 (RPA-T8) and CD62L (DREG-56) (all from BD Pharmingen) were used. The cells were then fixed in formaldehyde (4 %) and permeabilized in saponin buffer (0.5 %) (Sigma, USA) for 15 min. Finally, the cells were incubated with anti-TNF-α (PE) (L293) (BD Biosciences) washed and ressuspended in FACS buffer prior acquisition in flow cytometer.
Phenotypic analyses were performed by flow cytometry using a Becton Dickinson FACScalibur flow cytometer, collecting data on 7 × 104 lymphocytes gated according to their forward and side scatter properties. The sample acquisition and data analysis were performed using CellQuest software (BD Biosciences, USA).
Chaves A.T., de Assis Silva Gomes Estanislau J., Fiuza J.A., Carvalho A.T., Ferreira K.S., Fares R.C., Guimarães P.H., de Souza Fagundes E.M., Morato M.J., Fujiwara R.T., da Costa Rocha M.O, & Correa-Oliveira R. (2016). Immunoregulatory mechanisms in Chagas disease: modulation of apoptosis in T-cell mediated immune responses. BMC Infectious Diseases, 16, 191.
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4

Characterization of T Cell Subsets

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Mouse specific antibodies against CD3ε/γ (17A2; Biolegend), CD4 (RM4-5; eBioscience), CD25 (PC61.5; eBioscience), CD44 (IM7; BD Biosciences), CD62L (MEL-14; Life Technologies), CD126 (D7715A7; eBioscience), CD127 (25-1271-82; eBioscience),βTCR (H57-597), gp130 (125623; R&D Systems), IFNγ (XMG1.2; eBioscience), IL-4 (11B11; eBioscience), IL-17A (TC11-18H10.1; Biolegend), IL-21 (Recombinant mouse IL-21R Fc Chimera protein; R&D and IL-21 receptor antibody; Jackson Immuno Research) and PTPN2 (AF1930; R&D) were used. For detection of human antigens, we used antibodies specific to CD3 (UCHT1; BioLegend), CD4 (RPA-T4; eBioscience), CD45RA (HI100; BioLegend), CD45RO (UCHL1; BioLegend), CD62L (DREG-56; BD Biosciences), CD197 (CCR7; G043H7; BioLegend). Human and mouse cross-specific antibodies to pY STAT1 (pY701; 4a), pY-STAT3 (pY705; 4/P-STAT3), pS-STAT1 (pS727; K51-856) and pS STAT3 (pS727, 49/p-Stat3) were from BD Biosciences.
Twohig J.P., Figueras A.C., Andrews R., Wiede F., Cossins B.C., Soria A.D., Lewis M.J., Townsend M.J., Millrine D., Li J., Hill D.G., Fernandez J.U., Liu X., Szomolay B., Pepper C.J., Taylor P.R., Pitzalis C., Tiganis T., Williams N.M., Jones G.W, & Jones S.A. (2019). Activation of naïve CD4+ T cells re-tunes STAT1 signaling to deliver unique cytokine responses in memory CD4+ T cells. Nature immunology, 20(4), 458-470.
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5

NKT-cell Phenotyping and Characterization

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NKT-cell phenotype was assessed using monoclonal antibodies (mAbs) for CD3 (UCHT1), Vα24-Jα18 (6B11), CD4 (RPA-T4), granzyme B (GB11), CD62L (DREG-56; BD Biosciences, San Jose, CA), Vβ11 (C21; Beckman Coulter, Brea, CA), and IL-21R (17A12; BioLegend, ‎San Diego, CA and BD Biosciences). CD19-CAR expression by transduced NKTs was detected using anti-Id mAb (clone 136.20.1) (25 (link)), a gift from Dr. B. Jena (MD Anderson Cancer Center, Houston, TX). Intracellular staining was performed using a fixation/permeabilization solution kit (BD Biosciences) with mAbs for Bcl2 (N46–467; BD Biosciences) and BIM (Y36; Abcam, Cambridge, MA) followed by staining with a secondary goat anti-rabbit IgG-AF488 mAb (Abcam). Phosflow staining was performed using Cytofix buffer (BD Biosciences) and Perm buffer III (BD Biosciences) with mAb for Stat3 (pY705; Clone 4; BD Biosciences). Detection of Stat3 phosphorylation was performed after 15 minutes of treatment with IL-21. Fluorochrome- and isotype-matching antibodies suggested by BD Biosciences or R&D Systems were used as negative controls. Analysis was performed on an LSR-II 5-laser flow cytometer (BD Biosciences) using BD FACSDiva software version 6.0 and FlowJo 10.1 (Tree Star, Ashland, OR).
Ngai H., Tian G., Courtney A.N., Ravari S.B., Guo L., Liu B., Jin J., Shen E.T., Di Pierro E.J, & Metelitsa L.S. (2018). IL-21 selectively protects CD62L+ NKT cells and enhances their effector functions for adoptive immunotherapy. Journal of immunology (Baltimore, Md. : 1950), 201(7), 2141-2153.
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