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Natural unstained protein ladder 161 0317

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The Natural Unstained Protein Ladder (161-0317) is a pre-stained molecular weight standard used for protein size determination in SDS-PAGE. It consists of a mixture of naturally occurring proteins that have not been chemically stained. The ladder covers a wide range of molecular weights, allowing for accurate sizing of proteins during electrophoretic separation.

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Lab products found in correlation

M0630, by Merck Group (2 mentions) Horseradish peroxidase streptavidin sa 5004, by Vector Laboratories (2 mentions) Ez run prestained protein ladder bp3603, by Thermo Fisher Scientific (2 mentions) B2787, by Bio-Rad (2 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions) Thermomixer, by Eppendorf (2 mentions) Biotinyl asp glu val asp aldehyde, by Bachem (2 mentions)

2 protocols using natural unstained protein ladder 161 0317

1

Preparation and Analysis of Homocysteinylated Myoglobin

Cited 1 time
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Horseradish peroxidase streptavidin (SA-5004) was from Vector Laboratories (Burlingame, CA, USA). SuperSignal West Pico chemiluminescent substrate was from Thermo Scientific (Rockford, IL, USA). Myoglobin from equine skeletal muscle (M0630) was from Sigma-Aldrich. N-Homocysteinylated myoglobin was prepared as we previously reported [64 (link)]. Biotinyl-Asp-Glu-Val-Asp-aldehyde (CAS registry number: 178603-73-1) was from Bachem Americas (N-1470, Torrance, CA, USA; see Figure S1 for its structure in Support Information). Rhodamine-aldehyde (10 mM) in 50% 200-proof ethanol was synthesized as previously described [64 (link)]. EZ-Run prestained protein ladder (BP3603) was from Fisher Scientific. Natural unstained protein ladder (161-0317) was from Bio-Rad. Biotinylated protein markers were from Sigma-Aldrich (B2787) and Bio-Rad (161-0319), respectively. All reagents were ACS grade and used as received without further purification. All incubations were carried out in an Eppendorf Thermomixer® (Eppendorf North America, Hauppauge, NY, USA) at 25 °C unless specified otherwise.
Zang T., Pottenplackel L.P., Handy D.E., Loscalzo J., Dai S., Deth R.C., Zhou Z.S, & Ma J. (2016). Comparison of Protein N-Homocysteinylation in Rat Plasma under Elevated Homocysteine Using a Specific Chemical Labeling Method. Molecules, 21(9), 1195.
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2

Homocysteinylated Myoglobin Labeling Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Horseradish peroxidase streptavidin (SA-5004) was from Vector Laboratories (Burlingame, CA, USA). SuperSignal West Pico chemiluminescent substrate was from Thermo Scientific (Rockford, IL, USA). Myoglobin from equine skeletal muscle (M0630) was from Sigma-Aldrich. N-Homocysteinylated myoglobin was prepared as we previously reported [64 (link)]. Biotinyl-Asp-Glu-Val-Asp-aldehyde (CAS registry number: 178603-73-1) was from Bachem Americas (N-1470, Torrance, CA, USA; see Figure S1 for its structure in Support Information). Rhodamine-aldehyde (10 mM) in 50% 200-proof ethanol was synthesized as previously described [64 (link)]. EZ-Run prestained protein ladder (BP3603) was from Fisher Scientific. Natural unstained protein ladder (161-0317) was from Bio-Rad. Biotinylated protein markers were from Sigma-Aldrich (B2787) and Bio-Rad (161-0319), respectively. All reagents were ACS grade and used as received without further purification. All incubations were carried out in an Eppendorf Thermomixer® (Eppendorf North America, Hauppauge, NY, USA) at 25 °C unless specified otherwise.
Zang T., Pottenplackel L.P., Handy D.E., Loscalzo J., Dai S., Deth R.C., Zhou Z.S, & Ma J. (2016). Comparison of Protein N-Homocysteinylation in Rat Plasma under Elevated Homocysteine Using a Specific Chemical Labeling Method. Molecules, 21(9), 1195.
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