Tetracycline (TC), N-hydroxysuccinimide (NHS), 11-amino-1-undecanethiol, phosphate buffer solution (PBS) tablets, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), Ethanol, sodium dodecyl sulfate (SDS), Polyvinyl chloride (PVC), o-nitro phenyl octyl ether (NPOE), di-octyl phthalate (DOP), dibutyl phthalate (DBP), dibutyl sebacate (DBS), hydrochloric acid (HCl), diethyl ether, tetrahydrofuran (THF), di-octyl phthalate, bis(2-ethyl hexyl) sebacate were purchased from Sigma-Aldrich (France). The standard solutions and buffers were prepared with Millipore Milli-Q nanopure water (resistivity > 18 MW cm) which is produced by a Millipore Reagent Water System (France). Epoxy resin EPO TEK H70E 2LC was from Epoxy Technology, France. Cs[o-COSAN], was synthesized from 1,2-closo-C2B10H12 from Katchem Spol.sr.o (Kralupy nad Vltavou, Czech Republic), as reported in the literature [54 (link)]. The Na[o-COSAN] was obtained by means of cationic exchange resin from Cs[o-COSAN] following the previously described procedure [31 (link)].
Milli q nanopure water
Manufactured by Merck Group
Sourced in France
The Milli-Q nanopure water system is a laboratory water purification device that produces ultrapure water. It utilizes a multi-stage filtration process to remove impurities, resulting in water with a high level of purity and low levels of contaminants.
Automatically generated - may contain errors
Lab products found in correlation
N hydroxysuccinimide, by Merck Group (3 mentions)
Lambda2s spectrophotometer, by PerkinElmer (2 mentions)
Glutaraldehyde 2.5, by Merck Group (2 mentions)
N ethyl n 3 dimethylaminopropyl carbodiimide, by Merck Group (2 mentions)
N 2 hydroxyethylpiperazine n 2 ethanesulfonic acid, by Merck Group (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Ethanolamine, by Merck Group (2 mentions)
Phosphate buffer solution (pbs), by Merck Group (1 mentions)
Dioctyl phthalate, by Merck Group (1 mentions)
Dibutyl sebacate, by Merck Group (1 mentions)
Polyvinyl chloride, by Merck Group (1 mentions)
Dibutyl phthalate, by Merck Group (1 mentions)
Tetracycline, by Merck Group (1 mentions)
Bis 2 ethylhexyl sebacate, by Merck Group (1 mentions)
O nitrophenyloctylether, by Merck Group (1 mentions)
11 amino 1 undecanethiol, by Merck Group (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Tetrahydrofuran, by Merck Group (1 mentions)
Diethyl ether, by Merck Group (1 mentions)
7 protocols using milli q nanopure water
1
Electro-Analytical Sensor Fabrication
2
Electrochemical Characterization of Modified Electrodes
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All reagents were of analytical grade and were used without further purification. Millipore Milli-Q nanopure water (resistivity ≥ 18 MΩ cm) was used for the preparation of all solutions.
5-Sminosalicylic acid (5-ASA), acetaminophen (APAP), methylene blue (MB), choline chloride, ethylene glycol, potassium chloride, sodium monobasic phosphate, sodium dibasic phosphate, sodium hydroxide, acetic, boric, hydrochloric, perchloric, and phosphoric acid were purchased from Sigma-Aldrich (Darmstadt, Germany).
For the electrochemical characterization of the modified electrodes, the supporting electrolyte was 0.04 M Britton Robinson (BR) buffer solution, pH 7.0. All experiments were carried out at room temperature (25 ± 1 °C).
5-Sminosalicylic acid (5-ASA), acetaminophen (APAP), methylene blue (MB), choline chloride, ethylene glycol, potassium chloride, sodium monobasic phosphate, sodium dibasic phosphate, sodium hydroxide, acetic, boric, hydrochloric, perchloric, and phosphoric acid were purchased from Sigma-Aldrich (Darmstadt, Germany).
For the electrochemical characterization of the modified electrodes, the supporting electrolyte was 0.04 M Britton Robinson (BR) buffer solution, pH 7.0. All experiments were carried out at room temperature (25 ± 1 °C).
3
Electrochemical Detection of Biomarkers in Biological Fluids
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Nickel (II) nitrate hexahydrate (Ni(NO3)2 6H2O) and thiourea (CS(NH2)2) were acquired from Sigma Aldrich. Potassium hydroxide pellet (KOH) was obtained from SRL. Nitric acid (HNO3) was received from Rankem analytic. Glucose (Glu) (C6H12O6), ascorbic acid (C6H8O6) (AA), uric acid (C5H4N4O3) (UA), paracetamol (C8H9NO2) (PA), magnesium sulfate hexahydrate (MgSO4·6H2O), sodium chloride (NaCl), and calcium sulfate pentahydrate (CaSO4·5H2O) were obtained from Sigma Aldrich. Millipore Milli-Q nanopure water (resistivity ≥18 MΩ cm−1) system was used as the solvent throughout this work. All the reagents obtained and used in this work were of analytical grade, and were applied without further refinement. Human serum samples were provided by the SRM medical college hospital and the human urine samples were collected from healthy volunteers.
4
Biomolecular Conjugation Protocol
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Bovine serum albumin (purity over 98 %) was purchased from SIGMA, and its concentration was measured with a Perkin Elmer Lambda 2S spectrophotometer, using the standard molar absorption coefficient for Trp and Tyr at 280 nm (ε = 44,000 M -1 cm -1 ). Levothyroxine sodium pentahydrate, LT4 (888.93 g mol -1 ) solubilized in dimethyl sulfoxide (DMSO) to a stock solution of 2.8 mM was purchased from SIGMA. Glutaraldehyde 25 % (GA), ethanolamine 1 % (ETA), Nhydroxysuccinimide (NHS), N-ethyl-N3-dimethylaminopropyl carbodiimide (EDC), Cystamine (Cys) and N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) (HEPES ≥ 99.5 %) were also purchased from SIGMA. All samples were prepared in 0.1 M HEPES buffer, adjusted to pH 7.4 with a saturated NaOH solution. Millipore Milli-Qnanopure water (resistivity ≥ 18 MΩ cm) was used for the preparation of all solutions.
5
Proteasome Subunit Assay Protocol
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The reagents were of analytical grade and were used without further purification. Millipore Milli-Q nanopure water (resistivity ≥ 18 MΩ cm) was used for the preparation of all solutions.
Proteasome 20S (human) and 20S β5 subunit (human) monoclonal antibody (Abβ), 20S core subunits polyclonal antibody (Abcore) and 20S substrate carbobenzoxy(Z)-Leu(L)-Leu(L)-Glu(E)-7-amino-4-methylcoumarin (AMC) of caspase activity were from Enzo Life Sciences. The 4-mercaptophenylboronic acid, AmPhP, AmPh, Tris/HCl, KCl, NaCl, MgCl2, SDS, EDC, NHS, PBS with 0.1% Tween, and Fetal Bovine Serum with ≤10 EU/mL endotoxins were from Sigma-Aldrich. Alkaline Phosphatase Labeling Kit-NH2 was from Abnova (St. Louis, MO, USA). The labeling of Abcore with AlkP was done in agreement with the instructions in the labeling kit manual.
Stock solutions of Z-LLE-AMC, AmPh and AmPhP were prepared in dimethyl sulfoxide (DMSO). Stock solutions of 20S proteasome were prepared in water and kept at −20 °C until further utilization.
The proteasome assay buffer (PAB) pH = 7.5 contained 50 mM Tris/HCl, 25 mM KCl, 10 mM NaCl, 1 mM MgCl2 and 100 µM SDS.
Proteasome 20S (human) and 20S β5 subunit (human) monoclonal antibody (Abβ), 20S core subunits polyclonal antibody (Abcore) and 20S substrate carbobenzoxy(Z)-Leu(L)-Leu(L)-Glu(E)-7-amino-4-methylcoumarin (AMC) of caspase activity were from Enzo Life Sciences. The 4-mercaptophenylboronic acid, AmPhP, AmPh, Tris/HCl, KCl, NaCl, MgCl2, SDS, EDC, NHS, PBS with 0.1% Tween, and Fetal Bovine Serum with ≤10 EU/mL endotoxins were from Sigma-Aldrich. Alkaline Phosphatase Labeling Kit-NH2 was from Abnova (St. Louis, MO, USA). The labeling of Abcore with AlkP was done in agreement with the instructions in the labeling kit manual.
Stock solutions of Z-LLE-AMC, AmPh and AmPhP were prepared in dimethyl sulfoxide (DMSO). Stock solutions of 20S proteasome were prepared in water and kept at −20 °C until further utilization.
The proteasome assay buffer (PAB) pH = 7.5 contained 50 mM Tris/HCl, 25 mM KCl, 10 mM NaCl, 1 mM MgCl2 and 100 µM SDS.
6
Immunoassay Reagents and Materials
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TNF-α (Cat. No. 210-TA), mAb anti-TNF-α (Cat. No. MAB610), IL-10 (Cat. No. 1064-IL), sterile phosphate buffer saline solution (PBS) and PBS containing 0.1% bovine serum albumin (Cat. No. RB01 and RB02, respectively) were from R&D Systems (BioTechne, France). Hydrocortisone (cortisol, purity 99%, Cat. No. ab141250) was from abcam (France).
NT-proBNP (Cat. No. 8NT2) was from HyTest (Finland). Millipore Milli-Q nanopure water (resistivity > 18 MW cm) was produced by a Millipore Reagent Water System (France). 11triethoxysilyl undecanal (TESUD, 90%) was purchased from abcr (Germany). Pure ethanol (purity 95.0%) and sterile phosphate buffer saline (PBS) tablets were purchased from Sigma-Aldrich (France). PBS buffer used in this study was prepared by dissolving PBS tablets in the nanopure water as indicated by the supplier by yielding 0.01 M phosphate buffer (pH 7.4) containing 0.0027 M potassium chloride and 0.137 M sodium chloride. Epoxy resin EPO TEK H70E2LC was from Epoxy Technology (France).
NT-proBNP (Cat. No. 8NT2) was from HyTest (Finland). Millipore Milli-Q nanopure water (resistivity > 18 MW cm) was produced by a Millipore Reagent Water System (France). 11triethoxysilyl undecanal (TESUD, 90%) was purchased from abcr (Germany). Pure ethanol (purity 95.0%) and sterile phosphate buffer saline (PBS) tablets were purchased from Sigma-Aldrich (France). PBS buffer used in this study was prepared by dissolving PBS tablets in the nanopure water as indicated by the supplier by yielding 0.01 M phosphate buffer (pH 7.4) containing 0.0027 M potassium chloride and 0.137 M sodium chloride. Epoxy resin EPO TEK H70E2LC was from Epoxy Technology (France).
7
Biomolecular Conjugation Protocol
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Bovine serum albumin (purity over 98 %) was purchased from SIGMA, and its concentration was measured with a Perkin Elmer Lambda 2S spectrophotometer, using the standard molar absorption coefficient for Trp and Tyr at 280 nm (ε = 44,000 M -1 cm -1 ). Levothyroxine sodium pentahydrate, LT4 (888.93 g mol -1 ) solubilized in dimethyl sulfoxide (DMSO) to a stock solution of 2.8 mM was purchased from SIGMA. Glutaraldehyde 25 % (GA), ethanolamine 1 % (ETA), Nhydroxysuccinimide (NHS), N-ethyl-N3-dimethylaminopropyl carbodiimide (EDC), Cystamine (Cys) and N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) (HEPES ≥ 99.5 %) were also purchased from SIGMA. All samples were prepared in 0.1 M HEPES buffer, adjusted to pH 7.4 with a saturated NaOH solution. Millipore Milli-Qnanopure water (resistivity ≥ 18 MΩ cm) was used for the preparation of all solutions.
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