Primary antibodies used: Akt #9272 (1:1000), p-Akt (Ser473) # 4058 (1:1000), p-Akt (Thr308) #2965, Rab8A #6975 (1:1000), Rab10 #8127 (1:1000), AS160 #2670 (1:1000), p-AS160 (Thr642) #4288 (1:1000), IR beta #3025 (1:1000), p-IGF-IR beta (Tyr1131)/IR beta (Tyr1146) #3021 (1:1000) (all from Cell Signalling); LRRK2 (3514-1 (MJFF2) Epitomics); tubulin (ab6160, abcam); SLC2A4 (ARP43785_P050, avivasysbio, 1:100); GLUT4 (MAB1262, R&D Systems, 1:1000); Alexa Fluor 488 Mouse anti-β-Tubulin, Class III Clone TUJ1 #560338 (BD Pharmingen).
Secondary antibodies used: anti-rabbit IgG, HRP-linked #7074 (1:5000, Cell signalling); Goat anti-rat IgG, HRP-linked (1:20 000, ab6845, abcam); Rabbit anti-sheep IgG, HRP-linked (1:10 000, 31480, ThermoScientific); Goat anti-mouse IgG, HRP-linked (1:10 000, 172–1011, Bio-Rad); Cy3-conjugated Goat anti-rabbit IgG (1:350, 111-165-046, Jackson ImmunoResearch); Cy2-conjugated Goat anti-mouse IgG (1:350, 115-225-164, Jackson ImmunoResearch).
Secondary antibodies used: anti-rabbit IgG, HRP-linked #7074 (1:5000, Cell signalling); Goat anti-rat IgG, HRP-linked (1:20 000, ab6845, abcam); Rabbit anti-sheep IgG, HRP-linked (1:10 000, 31480, ThermoScientific); Goat anti-mouse IgG, HRP-linked (1:10 000, 172–1011, Bio-Rad); Cy3-conjugated Goat anti-rabbit IgG (1:350, 111-165-046, Jackson ImmunoResearch); Cy2-conjugated Goat anti-mouse IgG (1:350, 115-225-164, Jackson ImmunoResearch).