Z033429
The Z033429 is a laboratory instrument designed for conducting various analytical and testing procedures. It is a versatile piece of equipment that can be utilized in diverse research and testing applications. The core function of this product is to provide reliable and accurate measurements, data analysis, and sample processing capabilities to support scientific investigations and quality control processes. For detailed specifications and intended use, please refer to the product documentation or consult with an Agilent Technologies sales representative.
Lab products found in correlation
4 protocols using z033429
Immunofluorescence Staining of Brain Sections
Western Blot Analysis of Neuronal and Glial Markers
Immunohistochemical Staining of Neural Markers
Neurobehavioral Evaluation in BALB/c Mice
At day 3 after ICV injection, Motor Performance Test-Accelerating Rotating Rod Test was performed. The rotating rod apparatus was used to evaluate motor function. The mice were placed on the rod (3 cm in diameter) for four trials. Each trial lasted for a maximum of 5 min, during which the rotating rod underwent linear acceleration from 0 to 30 rpm and then remained at maximum speed. Animals were scored for their latency to fall (in seconds) in each trial.
Then the mice were deeply anesthetized and euthanized by transcardiac perfusion with fixative solution (4% paraformaldehyde and 2% glutaraldehyde in 0.1 M sodium cacodylate buffer, pH 7.4, 50 mL/animal). The brains were harvested and postfixed in 4% paraformaldehyde, dehydrated in increasing concentrations of sucrose (30–60%), infiltrated in OCT Tissue Freezing Medium (380148, Leica), and plastic-embedded. Frozen sections (15 μm) were cut, mounted on coverslips, and then dried at room temperature and stained with NeuN (ab128886, Abcam, 1:500) and GFAP (Z033429, Dako, 1:1000).
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