Glycerol 3 phosphate dehydrogenase α gdh

Unless otherwise specified, all reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA), including omeprazole. Luria-Bertani (LB) medium and isopropyl-β-D-thiogalactopyranoside (IPTG) were purchased from AMRESCO LLC (Cochran Road Solon, OH, USA). Glycerol-3-phosphate dehydrogenase (α-GDH) and reduced nicotinamide adenine dinucleotide (NADH) were purchased from Roche (Penzberg, Upper Bavaria, Germany). T7 oligonucleotides were from Novagen, and Phusion polymerase was from Thermo Scientific. Immobilized metal affinity chromatography (IMAC) resin was purchased from Bio-Rad (Hercules, California, USA). Amicon Ultra 30 kDa filters were purchased from Millipore Corporation (Billerica, Massachusetts, USA).

Unless otherwise specified, all reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA). Luria-Bertani (LB) medium and isopropyl-β-D-thiogalactopyranoside (IPTG) were purchased from AMRESCO LLC (Cochran Road Solon, OH, USA). Glycerol-3-phosphate dehydrogenase (α-GDH) and reduced nicotinamide adenine dinucleotide (NADH) were purchased from Roche (Penzberg, Upper Bavaria, Germany). Immobilized Metal Affinity Chromatography (IMAC) resin was purchased from Bio-Rad (Hercules, California, USA). Amicon Ultra 30 kDa filters were purchased from Millipore Corporation (Billerica, Massachusetts, USA).
The sulfhydryl reagents utilized were methylmethane thiosulfonate (MMTS), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB) and sodium (2-sulfonatoethyl) methanethiosulfonate (MTSES). The last was purchased from Toronto Research Chemicals (Toronto, Canada).
The commercially approved drugs used were omeprazole, rabeprazole and sulbutiamine. The last was purchased from Santa Cruz, Biotechnology (Dallas, Texas, USA).