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Anti ccr7 fitc clone 150503

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Anti-CCR7-FITC (clone 150503) is a fluorescently-labeled monoclonal antibody that binds to the chemokine receptor CCR7. It can be used for the detection and analysis of CCR7-expressing cells by flow cytometry.

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Lab products found in correlation

Facsdiva software version 6, by BD (2 mentions) Anti cd28 pe cy7 clone cd28.2, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

FITC-conjugated anti-CCR7 clone 150503 CCR7-FITC (150503) CCR7 (clone 150503, Anti-CCR7 (150503; Anti-CCR7 FITC CCR7-FITC Anti-CCR7

2 protocols using anti ccr7 fitc clone 150503

1

Quantifying CD4+ T Cell Subsets by Flow Cytometry

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CD4+ T lymphocyte subsets were determined by multi-channel flow cytometry for CD3, CD4, CD8, CD28, CD95, CCR5 and CCR7. CD4+ T lymphocyte counts were calculated by multiplying the total lymphocyte count by the percentage of CD3+ CD4+ T cells. Briefly, 100 µl of EDTA-anticoagulated whole blood was stained with anti-CD3-A700 (clone SP34.2), anti-CD4-PerCP Cy5.5 (clone L200), anti-CD8-APC H7 (clone SK1), anti-CCR5-PE (clone 3A9), anti-CD95 APC (clone DX2) all from BD Biosciences, anti-CD28-PE CY7 (clone CD28.2; eBiosciences), and anti-CCR7-FITC (clone 150503; R&D Systems). Fixed cells were collected (30,000 events) on a LSRII instrument using FACSDiva software version 6.1.1 (BD Biosciences) and data were analyzed using FlowJo Software (TreeStar, Ashland, OR).
Asmal M., Luedemann C., Lavine C.L., Mach L.V., Balachandran H., Brinkley C., Denny T.N., Lewis M.G., Anderson H., Pal R., Sok D., Le K., Pauthner M., Hahn B.H., Shaw G.M., Seaman M.S., Letvin N.L., Burton D.R., Sodroski J.G., Haynes B.F, & Santra S. (2014). Infection of Monkeys by Simian-human Immunodeficiency Viruses with Transmitted/ founder Clade C HIV-1 Envelopes. Virology, 475, 37-45.
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2

Multi-parameter Flow Cytometry Analysis of CD4+ T Cells

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CD4+ T lymphocyte subsets were determined by multi-channel flow cytometry for CD3, CD4, CD8, CD28, CD95, CCR5 and CCR7. CD4+ T lymphocyte counts were calculated by multiplying the total lymphocyte count by the percentage of CD3+ CD4+ T cells. Briefly, 100 μl of EDTA-anticoagulated whole blood was stained with anti-CD3-A700 (clone SP34.2), anti-CD4-PerCP Cy5.5 (clone L200), anti-CD8-APC H7 (clone SK1), anti-CCR5-PE (clone 3A9), anti-CD95 APC (clone DX2) all from BD Biosciences, anti-CD28-PE CY7 (clone CD28.2; eBiosciences), and anti-CCR7-FITC (clone 150503; R&D Systems). Fixed cells were collected (30,000 events) on a LSRII instrument using FACSDiva software version 6.1.1 (BD Biosciences) and data were analyzed using FlowJo Software (TreeStar, Ashland, OR).
Santra S., Tomaras G.D., Warrier R., Nicely N.I., Liao H.X., Pollara J., Liu P., Alam S.M., Zhang R., Cocklin S.L., Shen X., Duffy R., Xia S.M., Schutte R.J., Pemble IV C.W., Dennison S.M., Li H., Chao A., Vidnovic K., Evans A., Klein K., Kumar A., Robinson J., Landucci G., Forthal D.N., Montefiori D.C., Kaewkungwal J., Nitayaphan S., Pitisuttithum P., Rerks-Ngarm S., Robb M.L., Michael N.L., Kim J.H., Soderberg K.A., Giorgi E.E., Blair L., Korber B.T., Moog C., Shattock R.J., Letvin N.L., Schmitz J.E., Moody M.A., Gao F., Ferrari G., Shaw G.M, & Haynes B.F. (2015). Human Non-neutralizing HIV-1 Envelope Monoclonal Antibodies Limit the Number of Founder Viruses during SHIV Mucosal Infection in Rhesus Macaques. PLoS Pathogens, 11(8), e1005042.
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