Mda mb 231 human breast cancer htb 26 cell line

The MDA-MB-231 human breast cancer (HTB-26) cell line was purchased from ATCC (Manassas, VA, USA). The passage numbers of cells used in experiments ranged from 5 to 20. thapsigargin (10522) (TG), fura-2-acetoxymethyl ester (14591) (Fura-2), N,N′-[1,2-ethanediylbis[(oxy-2,1-phenylene)]]bis[N-[2-(acetyloxy)methoxyl]-2-oxoethyl]-bis[(acetyloxy)methyl] ester (BAPTA-AM-Acetoxymethyl ester) (15551) (BAPTA-AM), and trans-resveratrol (70675) (RES) were purchased from Cayman Chemical (Ann Arbor, MI, USA). U-73122 (J62898) and 2-Aminoethyl diphenylborinate (A16606) (2-APB) were purchased from Alfa Aesar (Ward Hill, MA, USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (AC158990050) (MTT) was purchased from Acros Organics (Morris Plains, NJ, USA). 4′-Butyrate resveratrol (BuRV), 4′-Isobutyrate resveratrol (IsoRV) and 4′-Pivalate resveratrol (PIV) were synthesized by the Andrus lab in the Department of Chemistry and Biochemistry at Brigham Young University [18 (link)].

Avenanthramides A, B, and C were purchased from Sigma Aldrich (St. Louis, MO) and stocks were diluted to 60 mM by DMSO. MDA-MB-231 human breast cancer (HTB-26) cell line was purchased from ATCC (Manassas, VA). The passage numbers of cells used in experiments ranged from 5 to 25. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was purchased from Acros Organics (Morris Plains, NJ). MTT solvent was made up in the lab using 4 mM HCl, 0.1% Nonidet P-4 (NP40) in isoproponol. Propidium iodide (PI) was purchased from Cayman Chemical (Ann Arbor, MI). FITC Annexin V Apoptosis Detection Kit (556547) was purchased from BD Biosciences (San Jose, CA). CellEvent Caspase 3/7 Green Flow Cytometry Assay Kit was purchased from Thermo Fisher Scientific (Waltham, MA) (C10427).