Anti wnt3a antibody

Manufactured by R&D Systems

Sourced in United States

The Anti-Wnt3a antibody is a laboratory tool used to detect and study the Wnt3a protein. Wnt3a is a secreted signaling protein that plays a role in various cellular processes. The antibody can be used in techniques such as Western blotting, immunohistochemistry, and ELISA to identify and quantify Wnt3a expression in biological samples.

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Lab products found in correlation

L cells, by American Type Culture Collection (1 mentions)

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Wnt3a (251 citations) Anti-Wnt3a (4 citations)

2 protocols using anti wnt3a antibody

Purification and Characterization of Wnt3a

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Wnt3a is a specific Wnt/β-catenin signaling agonist that can be efficiently recovered from conditioned medium from Wnt3a-secreting L-cells (Wnt3a-CM). Wnt3a purification was carried out, as previously described.^{84 (link),91 (link),92 (link)} The presence of the Wnt3a protein was detected with an anti- Wnt3a antibody (R & D Systems, Minneapolis, MN, USA). Purity was analyzed by SDS–PAGE (8%), stained with Coomassie Blue G250, and analyzed through densitometry by using software ImageJ.^{93 (link)}

Medina M.A., Andrade V.M., Caracci M.O., Avila M.E., Verdugo D.A., Vargas M.F., Ugarte G.D., Reyes A.E., Opazo C, & De Ferrari G.V. (2018). Wnt/β-catenin signaling stimulates the expression and synaptic clustering of the autism-associated Neuroligin 3 gene. Translational Psychiatry, 8, 45.

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Wnt3a Protein Purification Protocol

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Wnt3a is a specific Wnt/β-catenin signaling agonist that can be efficiently recovered from conditioned medium from Wnt3a-secreting L-cells (ATCC, Rockville, MD; see also the Wnt homepage at http://web.stanford.edu/group/nusselab/cgi-bin/wnt/). Wnt3a purification was carried out as previously described [28 (link), 57 (link), 58 (link)]. The presence of the Wnt3a protein was detected with an anti-Wnt3a antibody (R & D Systems, Minneapolis, MN). Purity was analyzed by SDS-PAGE (8%), stained with Coomassie Blue G250, and analyzed through densitometry by using software ImageJ [59 (link)].

Pérez-Palma E., Andrade V., Caracci M.O., Bustos B.I., Villaman C., Medina M.A., Ávila M.E., Ugarte G.D, & De Ferrari G.V. (2016). Early Transcriptional Changes Induced by Wnt/β-Catenin Signaling in Hippocampal Neurons. Neural Plasticity, 2016, 4672841.

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