Cd11b conjugated magnetic beads

Manufactured by Miltenyi Biotec

Sourced in Germany

CD11b-conjugated magnetic beads are a laboratory tool used for the isolation and enrichment of CD11b-positive cells from biological samples. The beads are coated with antibodies specific to the CD11b cell surface marker, allowing for the targeted separation and purification of CD11b-expressing cells. This product facilitates the study of these cells and their role in various biological processes.

Automatically generated - may contain errors

Lab products found in correlation

Hibernate medium, by Thermo Fisher Scientific (1 mentions) Dnase, by Roche (1 mentions) Collagenase, by Worthington (1 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions) 70 μm cell strainer, by Merck Group (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Collagenase d, by Thermo Fisher Scientific (1 mentions) Dmem f12, by Roche (1 mentions) Collagenase d, by Roche (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Percoll, by GE Healthcare (1 mentions) Trizol plus purification kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Magnetic beads (547 citations) CD11b (40 citations) CD11b beads (18 citations) Anti-CD11b conjugated magnetic beads (5 citations) CD11B- conjugated beads (3 citations)

3 protocols using cd11b conjugated magnetic beads

Isolation of Post-Mortem Microglia

Check if the same lab product or an alternative is used in the 5 most similar protocols

Microglia were isolated from post-mortem brain tissue as described previously^{39 (link)}. After autopsy, tissue was stored in Hibernate medium (Invitrogen, Carlsbad, CA, USA) at 4 °C until further processing. Microglia isolation started as soon as possible, at the latest after 24 h. A single-cell suspension was generated by mechanical and enzymatic digestion with collagenase (3700 units/mL; Worthington, USA) and DNase (200 µg/mL; Roche, Switzerland) for frontal lobe (GFM), temporal lobe (GTS) and thalamic (THA) tissues, or 0.2% trypsin and 30 mg DNase for subventricular zone (SVZ) tissue. A Percoll (Amersham, Merck, Germany) gradient was generated to separate viable cells from myelin, cellular debris, and erythrocytes. The middle layer was collected and washed twice, followed by positive selection of myeloid cells with CD11b-conjugated magnetic beads (Miltenyi Biotec, Germany) according to the manufacturer’s protocol. MACS-isolated CD11b⁺ cells were fixed with fixation/stabilization buffer (SmartTube) and frozen at −80 °C until analysis by mass cytometry^{39 (link)}.

Böttcher C., Fernández-Zapata C., Snijders G.J., Schlickeiser S., Sneeboer M.A., Kunkel D., De Witte L.D, & Priller J. (2020). Single-cell mass cytometry of microglia in major depressive disorder reveals a non-inflammatory phenotype with increased homeostatic marker expression. Translational Psychiatry, 10, 310.

+ Open protocol

+ Expand

Isolation of hepatic stellate and Kupffer cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The hepatic stellate cells and Kupffer cells were isolated as previously described^{47 (link)}. In particular, the suprahepatic inferior vena cava was tightened and the inferior vena cava was cannulated and perfused with 50 ml Hanks’ balanced salt solutions and then the portal vein was cut. The perfusion was continued with 50 ml Dulbecco’s modified Eagle medium F/12 (DMEM F/12; GibcoBRL) containing 12.5 mg pronase, followed by 50 ml DMEM F/12 containing 33.3 mg collagenase D (Roche). The liver was removed and agitated in 50 ml DMEM (GibcoBRL) containing 0.2 mg DNase I, 17 mg pronase, and 17 mg collagenase D at 42 °C for 10 min. The cell suspension was filtered through a 70 μm cell strainer (Sigma). The hepatic stellate cells were purified by 8.2% Nycodenz (Accurate Chemical and Scientific Corporation), and Kupffer cells were isolated from the non-parenchymal cell fraction by CD11b-conjugated magnetic beads (Miltenyi). The purity was confirmed by flow cytometry (Kupffer cells, ~ 83%; hepatic stellate cells, ~ 85%).

Shalapour S., Lin X.J., Bastian I.N., Brain J., Burt A.D., Aksenov A.A., Vrbanac A.F., Li W., Perkins A., Matsutani T., Zhong Z., Dhar D., Navas-Molina J.A., Xu J., Loomba R., Downes M., Yu R.T., Evans R.M., Dorrestein P.C., Knight R., Benner C., Anstee Q.M, & Karin M. (2017). Inflammation-induced IgA+ cells dismantle anti-liver cancer immunity. Nature, 551(7680), 340-345.

+ Open protocol

+ Expand

Microglia Enrichment from Mouse Brains

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

CSF1 OE or control mice (ages p14 or p15, n = 4 per group) were perfused with PBS following a fatal dose of pentobarbital sodium. Brains were isolated and bisected sagittally. Left brain hemispheres were formalin-fixed for other studies and microglia were enriched from the right hemisphere by pull-down utilizing CD11b-conjugated magnetic beads (Miltenyi Biotech) using published methods with the Percoll (GE Healthcare) method for myelin removal [5 (link)]. Cell pellets were suspended in TRIzol (Thermo Fisher) and RNA was purified using the TRIzol Plus Purification kit including an on-column DNAse digestion step (Thermo Fisher).

De I., Maklakova V., Litscher S., Boyd M.M., Klemm L.C., Wang Z., Kendziorski C, & Collier L.S. (2021). Microglial responses to CSF1 overexpression do not promote the expansion of other glial lineages. Journal of Neuroinflammation, 18, 162.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!