HS-PEG-NH2 (average molecular weight: 5 kDa), fluorescein isothiocyanate isomer I (FITC) and cyanine5.5 NHS ester (Cy5.5) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti HER2/neu peptides, AHNP (sequence: FCDGFYACYMDV), AHNP-PEG, FITC-labeled AHNP-PEG (FITC-AHNP-PEG) were custom-made by AnaSpec (Fremont, CA, USA). The p-SCN-Bn-DTPA was obtained from Macrocyclics (Plano, TX, USA).
Hs peg nh2
Manufactured by Merck Group
Sourced in United States
HS-PEG-NH2 is a heterofunctional polyethylene glycol (PEG) reagent with a succinimidyl ester group and a terminal amino group. It is used for the covalent attachment of PEG to molecules containing primary amines, such as proteins and peptides.
Automatically generated - may contain errors
Lab products found in correlation
Fluorescein isothiocyanate isomer 1 fitc, by Merck Group (1 mentions)
Cyanine5.5 nhs ester cy5.5, by Merck Group (1 mentions)
P scn bn dtpa, by Macrocyclics (1 mentions)
Protein lobind tubes, by Avantor (1 mentions)
Mpeg sh, by Merck Group (1 mentions)
Sodium borohydride, by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
Trisodium citrate, by Merck Group (1 mentions)
2 protocols using hs peg nh2
1
Synthesis of AHNP-PEG Conjugates
2
Synthesis and Functionalization of Immunogenic Adenovirus Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Reagents required for the preparation of the iAuNPs, including trisodium citrate, sodium borohydride, ascorbic acid, and CTAB, were purchased from Sigma-Aldrich. Hydrogen tetrachloroaurate (III) hydrate was obtained from Acros Organics. Poly(ethylene glycols) employed for the PEGylation (HS-PEG-NH2 with average Mn 2000 and mPEG-SH with average Mn 800) and glutaraldehyde were purchased from Sigma-Aldrich. Purified hexon protein from adenovirus type 5 (Adv5) (purified adenovirus type 5 hexon-liquid prepared in 10 mM bis-tris propane buffer, 375 mM NaCl at a concentration of 0.39 mg mL−1) was supplied by Bio-Rad. Protein LoBind tubes of 1.5, 2, and 15 mL from VWR were used for all experiments.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!