Prolong gold antifade reagent with 4 6 diamidine phenylindone dapi solution

McCoy’s 5A growth medium, phosphate buffered saline (PBS), and trypan blue were purchased from ATCC (Manassas, VA). Fetal bovine serum (FBS), penicillin/streptomycin, and 0.25% trypsin-EDTA (w/v) were purchased from Gibco (Grand Island, NY). Arsenic trioxide (lot # 02765-24) and Nunc TM Lab-TekTM chamber slides were purchased from Fisher Scientific (Houston, TX). A lipid peroxidation kit was purchased from Abcam (Cambridge, MA). Prolong Gold Antifade Reagent with 4′,6-diamidine phenylindone (DAPI) solution was purchased from Invitrogen Corporation (Grand Island, NY). ECL western blotting detection system reagents and Films (CL-X-posure) were purchased from Thermo Scientific/Pierce (Rockford, IL). Pre-cast gels were purchased from Fisher Scientific (Pittsburg, PA). The folin-phenol (DC) protein determination kit, cell lysis buffer, and non-fat milk were obtained from BioRad Laboratories (Hercules, CA) and, polyvinylidene difluoride (PVDF) membrane was obtained from Millipore (Bedford, MA). The primary antibodies anti-caspase 3 and anti-cytochrome c were purchased from Cell Signal Technology, Inc (Danvers, MA). The primary antibodies anti-Bcl-2, anti-Bax, and horseradish peroxidase (HRP) conjugated anti-mouse or anti-rabbit goat secondary antibodies and β-actin were purchased from EMD Millipore Corporation (Billerica, MA).