Nitrosamines and pentoxifylline were bought from Merck (Germany). Ether, Formalin, FRAP, sodium acetate, ferric chloride, Iron sulfate, hematoxylin-eosin, and zinc sulfate powder were purchased from Sigma (USA). Commercial kits were obtained from Pars Azmoon (I.R. Iran). All other buffer additives and solvents were obtained from Merck (Germany). pentoxifylline was dissolved in distilled water and diluted by normal saline (0.9%) to produce a stock solution from which different doses were prepared. The potency of the drug was confirmed by the manufacturer just prior to the initiation of this study. The stock solution was passed through a 0.45-mm pore size filter (Lida Manufacturing, Kenosha, Wis. USA). The drug was further diluted to the desired concentrations with normal saline. Nitrosamines, 200 μL from 1 g/mL stock solution in normal saline, was added to 19.8 mL of PBS to prepare 10 mg/mL nitrosamines solution for the injection.
Zinc sulfate powder
Manufactured by Merck Group
Sourced in United States
Zinc sulfate powder is a white, crystalline compound composed of zinc and sulfate ions. It is a common inorganic chemical compound with the chemical formula ZnSO4. Zinc sulfate powder is a versatile laboratory reagent used in a variety of applications.
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2 protocols using zinc sulfate powder
1
Nitrosamines and Pentoxifylline Preparation
2
Preparation of NIT Solution for Injection
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Ether, formalin, ferric-reducing ability of plasma (FRAP), sodium acetate, ferric chloride, iron sulfate, hematoxylin and eosin stain (HE), and zinc sulfate powder were purchased from Sigma Corporation (St Louis, Missouri, USA). Commercial kits were obtained from Pars Azmoon Company (I. R. Iran). NIT, PEN, and all other buffer additives and solvents were obtained from Merck (Honenbrunn, Germany). PEN was dissolved in distilled water and diluted by normal saline (0.9%) to prepare different experimental doses. The stock solution was passed through a 0.45-mm pore size filter (Lida Manufacturing, Kenosha, Wis. USA). NIT, 200 μL from 1 g/mL stock solution in normal saline, was added to 19.8 mL of phosphate-buffered saline to prepare 40 mg/mL NIT solution for the injection.
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