Hdac3 sirna

Manufactured by Santa Cruz Biotechnology

Sourced in United States

HDAC3 siRNA is a synthetic short interfering RNA (siRNA) designed to target and silence the expression of the HDAC3 gene. HDAC3 is a member of the histone deacetylase family and plays a role in regulating gene expression. The HDAC3 siRNA can be used as a research tool to study the biological functions of HDAC3 in various cellular and molecular processes.

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Lab products found in correlation

Pgl3 basic vector, by Promega (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Sirna transfection reagent, by Santa Cruz Biotechnology (1 mentions) Sirna transfection medium, by Santa Cruz Biotechnology (1 mentions) Trichostatin a, by Merck Group (1 mentions) Ms 275, by Selleck Chemicals (1 mentions) Hdac2 sirna, by Santa Cruz Biotechnology (1 mentions) Pci 34051, by Selleck Chemicals (1 mentions) Flag f3165 antibody, by Merck Group (1 mentions) Flag peptide, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Sybr select master mix, by Thermo Fisher Scientific (1 mentions) Mg132, by Merck Group (1 mentions) Caffeine, by Merck Group (1 mentions) Mouse and rabbit hrp conjugated antibodies, by Abcam (1 mentions)

Spelling variants of this product

SiRNAs (95 citations) HDAC3 (32 citations)

4 protocols using hdac3 sirna

Fgf21 and MR Promoter Cloning

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A 3-kb mouse Fgf21 promoter was amplified and cloned into pGL3-basic vector (Promega) to produce the 3-kb Fgf21-luciferase plasmid. Different fragments of Fgf21 promoter were cloned into pGL3-basic vector to yield various truncated Fgf21-luciferase plasmids. Similarly, a 2-kb mouse MR promoter was cloned into the pGL3-basic vector and produced the 2-kb MR-luciferase plasmid. The sequences of the primers used to amplify these promoters are listed in table S4.
HDAC3 siRNA, NCOR1 siRNA, and their respective control were purchased from Santa Cruz Biotechnology Inc. Primary hepatocytes were transfected with siRNA or control using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions.

Sun J.Y., Du L.J., Shi X.R., Zhang Y.Y., Liu Y., Wang Y.L., Chen B.Y., Liu T., Zhu H., Liu Y., Ruan C.C., Gan Z., Ying H., Yin Z., Gao P.J., Yan X., Li R.G, & Duan S.Z. (2023). An IL-6/STAT3/MR/FGF21 axis mediates heart-liver cross-talk after myocardial infarction. Science Advances, 9(14), eade4110.

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siRNA Knockdown of HDAC3 in Cells

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siRNA-mediated knockdown of HDAC3 was performed as per manufacturer’s instructions. Cells (2 × 10⁵) were seeded in each well of a six-well plate. After 18 h, cells were transfected with 80 pmol of control siRNA (Santa Cruz Biotechnology sc37007) and HDAC3 siRNA (Santa Cruz Biotechnology sc35539). Transfection was performed using siRNA transfection reagent (Santa Cruz Biotechnology sc29528) and siRNA transfection medium (Santa Cruz Biotechnology sc36868). After 24 h, the transfection medium was replaced with fresh culture medium, and the cells were further incubated for 48 h (72 h posttransfection). Cells were then used for compressive force assay.

Damodaran K., Venkatachalapathy S., Alisafaei F., Radhakrishnan A.V., Sharma Jokhun D., Shenoy V.B, & Shivashankar G.V. (2018). Compressive force induces reversible chromatin condensation and cell geometry–dependent transcriptional response. Molecular Biology of the Cell, 29(25), 3039-3051.

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Evaluating HDAC Inhibitor Effects

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Recombinant human TGF‐β1 (PHG9204) was purchased from Invitrogen (Waltham, MA, USA). Trichostatin A (TSA) was purchased from Sigma (T8552, St. Louis, MO, USA). MS275 and PCI34051 were purchased from Selleckchem (S1053 and S2012, respectively, Burlington, NC, USA). LMK235 was obtained from Prof. Thomas Kurz (Heinrich‐Heine Universität Düsseldorf, Germany) and synthesized according to a previously published protocol 15. To rule out the siRNA off‐target effect, we used siRNAs from two companies (Bioneer and Santa Cruz). siRNAs against HDAC1, HDAC2, and HDAC3 were purchased from Bioneer (Gyeonggi‐do, South Korea) and siRNAs against HDAC2, HDAC3, and HDAC8 were purchased from Santa Cruz Biotechnology (Dallas, TX, USA); HDAC2 siRNA (sc‐29345), HDAC3 siRNA (sc‐35538), and HDAC8 siRNA (sc‐35548).

Choi S.Y., Kee H.J., Kurz T., Hansen F.K., Ryu Y., Kim G.R., Lin M.Q., Jin L., Piao Z.H, & Jeong M.H. (2016). Class I HDACs specifically regulate E‐cadherin expression in human renal epithelial cells. Journal of Cellular and Molecular Medicine, 20(12), 2289-2298.

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Characterization of HIV-1 Vpr Interacting Proteins

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HDAC1 (ab7028), HDAC2 (ab7029), HDAC3 (ab32369), HDAC8 (ab18968), histone H3 (ab70550), and HIV-1 Tat (ab43014) antibodies were purchased from Abcam. VprBP (A301-888A) antibody was from Bethyl Laboratories. Flag antibody (F3165) was from Sigma-Aldrich. GAPDH (14C10) and rabbit IgG isotype control (2729) were from Cell Signaling Technology. Anti-p24-PE (sc-69728 PE) was from Santa Cruz. Ace H3K9 (39917) and Ace H4K5 (39699) antibodies were from active motif. Mouse and rabbit HRP-conjugated antibodies were from Abcam. Monoclonal antibody against a conserved region of HIV-1 Vpr was raised in mouse by conjugating KLH (keyhole limpet hemocyanin) to a synthetic peptide (EAVRHFPRHWLHGLGQ) corresponding to amino acids 29–44 of Vpr prevalent among clinical isolates. SYBR Select Master Mix was from Life Technologies. Benzonase nuclease was from Novagen. Caffeine, Flag peptides, SAHA, and DMSO were from Sigma-Aldrich. MG132 was from Millipore. VprBP and non-targeting siRNAs were from Dharmacon. HDAC1 siRNA was from Qiagen. HDAC3 siRNA was from Santa Cruz Biotechnology. HDAC-Glo™ I/II Reagent was from Promega.

Romani B., Kamali Jamil R., Hamidi-Fard M., Rahimi P., Momen S.B., Aghasadeghi M.R, & Allahbakhshi E. (2016). HIV-1 Vpr reactivates latent HIV-1 provirus by inducing depletion of class I HDACs on chromatin. Scientific Reports, 6, 31924.

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