Cell viability was initially assessed using Alamar Blue (AbD Serotec) according to the manufacturer’s instructions. Cell counting using a hemocytometer was also undertaken.
Apoptosis was examined using the Annexin V-FITC apoptosis detection kit (Trevigen) according to manufacturer’s instructions. Cleaved PARP was measured in cells fixed in 4% paraformaldehyde using Alexa Fluor 647 Conjugate (BD Biosciences). Analyzes were performed by flow cytometry using a FACS Canto II (BD Biosciences). Assessment of activated caspase was made on cells fixed and permeabilized using a Leucoperm kit (AbD Serotec), active caspase 3 was measured using PE-conjugated polyclonal rabbit anti-active caspase-3 (BD Pharmingen).
Apoptosis was examined using the Annexin V-FITC apoptosis detection kit (Trevigen) according to manufacturer’s instructions. Cleaved PARP was measured in cells fixed in 4% paraformaldehyde using Alexa Fluor 647 Conjugate (BD Biosciences). Analyzes were performed by flow cytometry using a FACS Canto II (BD Biosciences). Assessment of activated caspase was made on cells fixed and permeabilized using a Leucoperm kit (AbD Serotec), active caspase 3 was measured using PE-conjugated polyclonal rabbit anti-active caspase-3 (BD Pharmingen).