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2 protocols using porcine pancreatic α amylase

1

Enzymatic Inhibition Assay Protocol

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Rat intestinal α-glucosidase and porcine pancreatic α-amylase were procured from Solarbio, China. Hydrogen peroxide, butylated hydroxytoluene (BHT), acarbose, and para-nitrophenyl-glucopyranoside (ρNPG) were obtained from Sigma-Aldrich, USA. Amylose, other chemicals, and reagents used were of analytical grade.
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2

Bioactive Compounds: Extraction and Evaluation

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Gallic acid (GA), catechin (C), epicatechin (EC), gallocatechin (GC), epigallocatechin (EGC), epicatechin-3-O-gallate (ECG,), epigallocatechin gallate (EGCG), epicatechin gallate (ECG), gallocatechin gallate (GCG and ellagic acid (EA) were purchased from Sigma-Aldrich (Saint Louis, MO, USA) with a purity of > 98 %. Folin-Ciocalteu phenol reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,4,6-tri-(2-pyridyl)-1,3,5-triazine (TPTZ), dinitrosalicylic acid (DNS), 4-nitrophenyl-a-d-glucopyranoside (pNPG), porcine pancreatic α-amylase (10 U/mg), Saccharomyces cerevisiae α-glucosidase (100 U/mg), porcine pancreatic lipase (30 U/mg) and porcine pancreatic cholesterol esterase (100 U/mg) were obtained from Beijing Solarbio Science & Technology Co. Ltd. (Beijing, China). All other reagents were of analytical grade and were commercially available.
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