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Ac caspase 3 monoclonal antibody

Manufactured by Cell Signaling Technology
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The Ac-caspase-3 monoclonal antibody is a laboratory tool used to detect the presence of activated caspase-3, a key enzyme involved in the apoptosis (programmed cell death) pathway. This antibody specifically recognizes the cleaved, active form of caspase-3, allowing researchers to study and monitor cell death processes.

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Lab products found in correlation

Lionheart microscope, by Agilent Technologies (2 mentions)

2 protocols using ac caspase 3 monoclonal antibody

1

Immunofluorescence Staining of Activated Caspase-3

Check if the same lab product or an alternative is used in the 5 most similar protocols
Frozen sections (5 μm) were fixed with 4% paraformaldehyde-PBS 20 min at room temperature, permeabilized with 0.3% Triton X-100 in 1X PBS, blocked in 3% BSA (or 10% FBS) in 1X PBS, stained with primary antibodies for overnight at 4°C, followed by staining with 2° antibodies (1 hr) and DAPI stain. Samples were covered with coverglasses and imaged using Lionheart microscope (Biotek). Ac-caspase-3 monoclonal antibody (9661S, Cell Signaling, 1:200) was used to perform the immunofluorescence staining.
Wang W., Wu S., Cen Z., Zhang Y., Chen Y., Huang Y., Cillo A.R., Prokopec J.S., Quarato G., Vignali D.A., Stewart-Ornstein J., Li S., Lu B, & Gong Y.N. (2022). Mobilizing phospholipids on tumor plasma membrane implicates phosphatidylserine externalization blockade for cancer immunotherapy. Cell reports, 41(5), 111582.
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2

Immunofluorescence Staining of Activated Caspase-3

Check if the same lab product or an alternative is used in the 5 most similar protocols
Frozen sections (5 μm) were fixed with 4% paraformaldehyde-PBS 20 min at room temperature, permeabilized with 0.3% Triton X-100 in 1X PBS, blocked in 3% BSA (or 10% FBS) in 1X PBS, stained with primary antibodies for overnight at 4°C, followed by staining with 2° antibodies (1 hr) and DAPI stain. Samples were covered with coverglasses and imaged using Lionheart microscope (Biotek). Ac-caspase-3 monoclonal antibody (9661S, Cell Signaling, 1:200) was used to perform the immunofluorescence staining.
Wang W., Wu S., Cen Z., Zhang Y., Chen Y., Huang Y., Cillo A.R., Prokopec J.S., Quarato G., Vignali D.A., Stewart-Ornstein J., Li S., Lu B, & Gong Y.N. (2022). Mobilizing phospholipids on tumor plasma membrane implicates phosphatidylserine externalization blockade for cancer immunotherapy. Cell reports, 41(5), 111582.
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