Primary hippocampal neurons and HT22 cells in culture dishes were randomly exposed to 3.4% sevoflurane in fresh gas (5% CO2, 21% O2, and balanced N2) for 5 h (group S) or only to fresh gas (group C) at 37°C in a chamber (RWD Life Science, Shenzhen, China). A steady concentration of 3.4% sevoflurane was kept during the experiments by a Capnomac gas monitor (Datex-Ohmeda, Helsinki, Finland).
Capnomac gas monitor
Manufactured by GE Healthcare
Sourced in Finland
The Capnomac gas monitor is a medical device used to measure the concentration of carbon dioxide (CO2) in a patient's exhaled breath. It provides a real-time display of the carbon dioxide levels, which is a critical parameter for monitoring a patient's respiratory function.
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Lab products found in correlation
2 protocols using capnomac gas monitor
1
Sevoflurane Exposure on Neuronal Cells
2
Sevoflurane Effect on Cortical Neurons
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HCN-2 human cortical neuron cells were purchased from ATCC (ATCC, Rockville, MD, USA) and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 15% fetal bovine serum (Invitrogen, Shanghai, China) in an incubator under 5% CO2 at 37°C. HCN-2 cell culture dishes were randomly divided between the experimental and control groups (n=6 each). The culture dishes were placed in an anesthesia induction chamber maintained at 37°C (RWD Life Science Co., Ltd., Shenzhen, China) and containing fresh gas (21% O2, 5% CO2, remainder N2) alone for the control group and with 3.4% sevoflurane for the experimental group (group S) for six hours. This sevoflurane concentration was steadily maintained throughout the experiments using a Capnomac gas monitor (Datex-Ohmeda, Helsinki, Finland).
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