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Ibeam smart 200 mw

Manufactured by Spectra-Physics
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The IBeam smart 200 mW is a compact, high-performance laser module that delivers a stable, continuous-wave laser beam with a power output of 200 milliwatts. The laser module features a solid-state laser source and integrated electronics for precise power and beam control.

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Lab products found in correlation

Apochromat na1, by Zeiss (2 mentions) Ixon du 897 dv em ccd camera, by Oxford Instruments (2 mentions) Axiovert 200, by Zeiss (2 mentions) Optosplit, by Cairn Research (2 mentions)

2 protocols using ibeam smart 200 mw

1

Multi-Color Microscopy Setup and Techniques

Check if the same lab product or an alternative is used in the 5 most similar protocols
All experiments were performed on a modified Zeiss Axiovert 200 inverted microscope equipped with a 100 × oil-immersion objective (Zeiss Apochromat NA1.46). The setup was equipped with a 640 nm diode laser (Toptica iBeam smart 200 mW), a 532 nm diode-pumped solid state (DPSS) laser (Spectra physics Millennia 6s) and a near UV light 405 nm ion laser (Coherent Innova 90C). Intensity modulation and timings were controlled either directly or with an acousto-optic modulator (AOM) using custom-written Labview software. Laser lines were overlaid with an OBIS Galaxy beam combiner (Coherent). Emission light was filtered using appropriate filter sets (Chroma) and recorded on an IXON DU 897-DV EM-CCD camera (Andor). Multi-color imaging was performed using an emission light splitter (Optosplit; Cairn Research) adapted to the spectral characteristics of the used fluorophores. Total internal reflection fluorescence (TIRF) illumination was achieved by shifting the excitation beam in parallel to the optical axis with a mirror mounted on a motorized movable table. For ratiometric Fura-2 imaging, we used a polychromatic Xenon light source combined with a monochromator (polychrome V; TILL photonics) that provided light at 340 nm and 380 nm.
Baumgart F., Arnold A., Leskovar K., Staszek K., Fölser M., Weghuber J., Stockinger H, & Schütz G.J. (2016). Varying label density allows artifact-free analysis of membrane-protein nanoclusters. Nature methods, 13(8), 661-664.
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2

Multi-Color Microscopy Setup and Techniques

Check if the same lab product or an alternative is used in the 5 most similar protocols
All experiments were performed on a modified Zeiss Axiovert 200 inverted microscope equipped with a 100 × oil-immersion objective (Zeiss Apochromat NA1.46). The setup was equipped with a 640 nm diode laser (Toptica iBeam smart 200 mW), a 532 nm diode-pumped solid state (DPSS) laser (Spectra physics Millennia 6s) and a near UV light 405 nm ion laser (Coherent Innova 90C). Intensity modulation and timings were controlled either directly or with an acousto-optic modulator (AOM) using custom-written Labview software. Laser lines were overlaid with an OBIS Galaxy beam combiner (Coherent). Emission light was filtered using appropriate filter sets (Chroma) and recorded on an IXON DU 897-DV EM-CCD camera (Andor). Multi-color imaging was performed using an emission light splitter (Optosplit; Cairn Research) adapted to the spectral characteristics of the used fluorophores. Total internal reflection fluorescence (TIRF) illumination was achieved by shifting the excitation beam in parallel to the optical axis with a mirror mounted on a motorized movable table. For ratiometric Fura-2 imaging, we used a polychromatic Xenon light source combined with a monochromator (polychrome V; TILL photonics) that provided light at 340 nm and 380 nm.
Baumgart F., Arnold A., Leskovar K., Staszek K., Fölser M., Weghuber J., Stockinger H, & Schütz G.J. (2016). Varying label density allows artifact-free analysis of membrane-protein nanoclusters. Nature methods, 13(8), 661-664.
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