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Mmp 2 9

Manufactured by Cell Signaling Technology
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MMP-2/-9 is a laboratory product that can be used to detect and measure the activity of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) enzymes. These enzymes are involved in the breakdown of extracellular matrix components and play a role in various biological processes.

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Lab products found in correlation

E cadherin, by Cell Signaling Technology (4 mentions) Snail, by Cell Signaling Technology (3 mentions) Caspase 3, by Cell Signaling Technology (2 mentions) Eif4e, by Cell Signaling Technology (2 mentions) P eif4e, by Cell Signaling Technology (2 mentions) Mnk1 2, by Cell Signaling Technology (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions) Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) N cadherin, by Cell Signaling Technology (2 mentions) β actin, by Cell Signaling Technology (2 mentions) α tubulin, by Cell Signaling Technology (2 mentions) Ripa buffer, by Thermo Fisher Scientific (2 mentions) Gemcitabine, by Merck Group (1 mentions) Nanog, by Cell Signaling Technology (1 mentions) β catenin, by Thermo Fisher Scientific (1 mentions) Cdc42, by Santa Cruz Biotechnology (1 mentions) Cyclin b1, by Santa Cruz Biotechnology (1 mentions) Cox 2, by Thermo Fisher Scientific (1 mentions)

4 protocols using mmp 2 9

1

Synthesis and Evaluation of Galeterone Analogs

Cited 1 time
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Galeterone and analogs (VNPT55, VNPP414 and VNPP433-3β) were designed and synthesized in our laboratory [26 (link), 68 (link), 69 ] and dissolved in DMSO. Gemcitabine was purchased from Sigma Aldrich. CGP-57380 was purchased from Eli Lilly. Cell culture reagents (FBS, RPMI, and DMEM) were from Invitrogen. β-catenin, Cox-2, Oct-4, Nanog, β-actin, β-Tubulin, Gapdh, Mnk1/2, eIF4E, peIF4E, N-cadherin, E-cadherin, Snail, Slug, MMP-2/-9, BMI-1, NF-κB (p65, p52), caspase 3, PARP anti-mouse and anti-rabbit HRP were purchased from cell signaling. C-Myc, Bax, Bcl-2, K-RAS, CXCR4, cyclin B1, cyclin D1, CDC42 and EZH2 antibodies were purchased from Santa Cruz biotech.
Kwegyir-Afful A.K., Murigi F.N., Purushottamachar P., Ramamurthy V.P., Martin M.S, & Njar V.C. (2016). Galeterone and its analogs inhibit Mnk-eIF4E axis, synergize with gemcitabine, impede pancreatic cancer cell migration, invasion and proliferation and inhibit tumor growth in mice. Oncotarget, 8(32), 52381-52402.
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2

Western Blot Analysis of Apoptotic Markers

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The following antibodies were purchased (Supplementary Table S1): primary antibodies to homeobox B9 (HOXB9, Thermo Fisher Scientific, Waltham, MA, USA), matrix metalloproteinase-2, -9 (MMP-2, -9, Cell Signaling Technology, Danvers, MA, USA), B-cell lymphoma 2 (BCL-2, abCAM, Cambridge, UK), BCL2-associated X (BAX, abCAM, Cambridge, UK), E-Cadherin, Survivin, Caspase-3, Cleaved Caspase-3, Cleaved Caspase-9, PARP, Cleaved PARP (Cell Signaling Technology, Danvers, MA, USA), excision repair cross-complementation-1 (ERCC-1, abCAM, Cambridge, UK), multidrug resistance-associated protein 2 (MRP-2, abCAM, Cambridge, UK), X-linked inhibitor of apoptosis protein (XIAP, Cell Signaling Technology, Danvers, MA, USA) and α-tubulin (Cell Signaling Technology, Danvers, MA, USA). The anti-rabbit IgG and anti-mouse IgG HRP (Horseradish Peroxidase)-conjugated secondary antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). Cisplatin was purchased from Selleckchem (Houston, TX, USA).
Suh D.H., Park W.H., Kim M., Kim K., No J.H, & Kim Y.B. (2023). HOXB9 Overexpression Confers Chemoresistance to Ovarian Cancer Cells by Inducing ERCC-1, MRP-2, and XIAP. International Journal of Molecular Sciences, 24(2), 1249.
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3

Protein Expression Analysis of Cell Signaling

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Cells were lysed in RIPA buffer (Thermo Fisher Scientific, Rochester, NT, USA) containing 25 mM Tris-HCl (pH 7.6), 150 mM NaCl, 1.0% nonidet-P 40 (NP40), 1.0% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS) with protease inhibitor cocktail and PhosphoSTOP (Roche Applied Science, Vienna, Austria, pH 7.4), and prepared as described previously4 (link). The following antibodies were used for Western blotting analysis: anti-phospho-p65 (NF-κB), -phospho-IκBα, -phospho-p53 (Ser 15, 20, and 46), -phospho-EGFR, -E-cadherin, -vimentin, -Slug, -Snail, -MMP2/9, and α-tubulin (Cell Signaling Technology, Danvers, MA, 1:1000).
Chang J.W., Kang S.U., Shin Y.S., Seo S.J., Kim Y.S., Yang S.S., Lee J.S., Moon E., Lee K, & Kim C.H. (2015). Combination of NTP with cetuximab inhibited invasion/migration of cetuximab-resistant OSCC cells: Involvement of NF-κB signaling. Scientific Reports, 5, 18208.
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4

Prostate Cancer Cell Line Maintenance and Analysis

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Human PC cells lines CWR22Rv1, LNCaP, PC-3 and DU145 PC cells were maintained in RPMI supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. Gal/VNPT55 were synthesized in our laboratory [70 (link)] and dissolved in DMSO. Cell culture reagents (FBS, RPMI, and DMEM) were from Invitrogen (Carlsbad, California, USA). U0126, β-actin, Gapdh, Mnk1/2, eIF4E, peIF4E, N-Cadherin, E-Cadherin, Snail, Slug, MMP-2/-9, BMI-1 anti-mouse and anti-rabbit horseradish peroxide (HRP) were purchased from cell signaling (Danvers, Massachusetts, USA). CGP-57380 was purchased from Sigma Aldrich (St. Louis, Missouri, USA). Twist1 polyclonal, EZH2, RhoA, Oct-4 and Nanog antibodies were from Santa Cruz (Dallas, Texas, USA) and the mouse monoclonal antibody from Abcam. β-Catenin, CD44 and VEGF were from BIOSS (Woburn, Massachusetts, USA).
Treated cells were lysed with radioimmunoprecipitation assay (RIPA) buffer (Sigma), supplemented with protease inhibitors (Roche, Indianapolis, Indiana, USA), 1 mM EDTA and 1 mM PMSF (Sigma). Western blotting was done as previously described [58 ].
Kwegyir-Afful A.K., Bruno R.D., Purushottamachar P., Murigi F.N, & Njar V.C. (2016). Galeterone and VNPT55 disrupt Mnk-eIF4E to inhibit prostate cancer cell migration and invasion. The FEBS journal, 283(21), 3898-3918.
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