Nqo1 antibody

RPMI 1640 and fetal bovine serum were purchased from Gibco-BRL (Gibco-BRL, Carlsbad, California, USA). Active oxygen detection kit (S0033), total SOD active detection kit (S0101), and lipid oxidation detection kit (S0131) were purchased from Full-Style Gold (Beijing, China). The enhanced BCA protein kit (P0009) and nuclear cytoplasmic protein extraction kit (P0028) were purchased from Beyotime Biotechnology (Shanghai, China). Keap1 antibody (catalog number 60027-1-lg), Nrf2 antibody (catalog number 66504-1-lg), NQO1 antibody (catalog number 67240-1-lg), and HO-1 antibody (catalog number 67643-1-lg) are available for purchase from Proteintech (USA). Nrf2 (phospho S40) (catalog number EP1809Y) was purchased from Abcam (UK). β-Actin (Cat. No. 12620), anti-rabbit IgG HRP conjugate (V7951), and anti-mouse IgG HRP conjugate (W4021) were purchased from Promega Corporation (Madison, W1, USA). The inhibitor ML385 (catalog number HY-100523) was purchased from MCE (Shanghai, China). All other reagents for this study were of analytical grade.

DMEM/F12 media, fetal bovine serum, and insulin-transferrin-selenium solution were purchased from Gibco (Beijing, China). SEPP1 antibody was purchased from Abcam (HKSP, N.T. Hong Kong, China). Nrf2 antibody was purchased from Abclonal (Wuhan, China). GPx1 antibody was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). GAPDH antibody was purchased from Servicebio (Wuhan, China). PGC-1α antibody, HIS3 antibody, and NQO1 antibody were purchased from Proteintech Group, Inc. (Wuhan, China). Mito Tracker green, Reactive Oxygen Species Assay Kit, and Cell Counting Kit-8 were purchased from Beyotime (Shanghai, China). JC-1 mitochondrial staining kit was purchased from Biosharp (Shanghai, China). Annexin V-FITC/PI Apoptosis Detection kit was purchased from YEASEN (Shanghai, China). Sodium selenite (Na₂SeO₃) was purchased from sigma (Shanghai, China). L-Se-methylselenocysteine (L-SeMC) was purchased from Macklin (Shanghai, China). Palmitic acid (PA) was purchased from sigma (Shanghai, China). Dexamethasone was purchased from Solarbio (Beijing, China). Selenium-enriched Spirulina (Se content 350 mg/kg) was provided by Professor Kaiyao Huang from the Institute of Hydrobiology, Chinese Academy of Sciences (Wuhan, China). Hematoxylin and Eosin Staining Kit was purchased from Beyotime (Shanghai, China). Masson Stain Kit was purchased from YEASEN (Shanghai, China).