Cd1c bdca1 percp cy5

Manufactured by BioLegend

CD1c/BDCA1-PerCp-Cy5.5 is a fluorophore-conjugated monoclonal antibody that binds to the CD1c (BDCA-1) surface antigen. CD1c is expressed on a subset of dendritic cells and can be used to identify and isolate this cell population.

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Lab products found in correlation

Facsaria 2, by BD (2 mentions) Diva software, by BD (2 mentions) Cd11c v450 b ly6, by BD (2 mentions) Hla dr apc eflour780 ln3, by Thermo Fisher Scientific (2 mentions) Human pan dc pre enrichment kit, by STEMCELL (2 mentions) Lineage cocktail 1 fitc, by BD (2 mentions)

Spelling variants of this product

PerCP-Cy5.5 (133 citations)

2 protocols using cd1c bdca1 percp cy5

Isolation and Sorting of Human Blood Dendritic Cells

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Human blood DCs were isolated from PBMCs using leukapheresis products. DCs were first enriched using human Pan-DC Pre-Enrichment Kit (StemCell Technologies) and then stained with fluorochrome-conjugated specific antibodies including Lineage cocktail 1-FITC (BD, dilution: 1:10), CD11c-V450 (B-ly6, BD, dilution: 1:50), CD1c/BDCA1-PerCp-Cy5.5 (L161, Biolegend, dilution 1:25), HLA-DR-APC-eFlour780 (LN3, eBioscience, dilution: 1:50), CD303/BDCA-2-PE (AC144, Mitenyi Biotec, dilution: 1:25) and CD141/BDCA3-APC (AD5-14H12, Mitenyi Biotec, dilution: 1:25). After washing with PBS, DCs were sorted as Lineage-HLA-DR+CD11c+ cells differentially expressing CD1c and CD141 using FACSAria II with Diva software (BD).

Banchereau R., Baldwin N., Cepika A.M., Athale S., Xue Y., Yu C.I., Metang P., Cheruku A., Berthier I., Gayet I., Wang Y., Ohouo M., Snipes L., Xu H., Obermoser G., Blankenship D., Oh S., Ramilo O., Chaussabel D., Banchereau J., Palucka K, & Pascual V. (2014). Transcriptional specialization of human dendritic cell subsets in response to microbial vaccines. Nature Communications, 5, 5283.

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Isolation and Sorting of Human Blood Dendritic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human blood DCs were isolated from PBMCs using leukapheresis products. DCs were first enriched using human Pan-DC Pre-Enrichment Kit (StemCell Technologies) and then stained with fluorochrome-conjugated specific antibodies including Lineage cocktail 1-FITC (BD, dilution: 1:10), CD11c-V450 (B-ly6, BD, dilution: 1:50), CD1c/BDCA1-PerCpCy5.5 (L161, Biolegend, dilution 1:25), HLA-DR-APC-eFlour780 (LN3, eBioscience, dilution: 1:50), CD303/BDCA-2-PE (AC144, Mitenyi Biotec, dilution: 1:25) and CD141/BDCA3-APC (AD5-14H12, Mitenyi Biotec, dilution: 1:25). After washing with PBS, DCs were sorted as Lineage-HLA-DR+CD11c+ cells differentially expressing CD1c and CD141 using FACSAria II with Diva software (BD).

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