Hela cell lines

Deionized water (resistivity: 18.2 MΩ cm⁻¹), which was obtained from a Milli-Q water purification system, was utilized for the preparation of all aqueous solutions. Hydrogen tetrachloroaurate(III) tetrahydrate (HAuCl₄·4H₂O, Nacalai Tesque), trisodium citrate dihydrate (Kanto Chemical, Japan), L-ascorbic acid (Fujifilm Wako Pure Chemical, Japan), palladium(II) chloride (PdCl₂, Fujifilm Wako Pure Chemical, Japan), hydrochloric acid (HCl, Fujifilm Wako Pure Chemical, Japan), hydroxypropyl cellulose (Sigma-Aldrich, USA), 2-propanol (Kishida Chemical, Japan), ammonium aqueous solution (28 wt%, Kishida Chemical, Japan), titanium diisopropoxide bis(acetylacetonate) (TDAA, Sigma-Aldrich, USA), and 8-ArmPEG-Amine (20,000 Da, Biopharma PEG Scientific, USA) were used as received. Dulbecco's Modified Eagle's Medium (D-MEM, low glucose) with L-glutamine and phenol red and PBS(-) were obtained from Fujifilm Wako Pure Chemical, Japan. Penicillin streptomycin, trypsin–EDTA (0.25%), and fetal bovine serum (FBS) were obtained from Gibco, USA. HeLa cell lines were purchased from JCRB Cell Bank, Japan.

HeLa cell lines were obtained from the Japanese Collection of Research Bioresources (JCRB) Cell Bank. SiHa cells were obtained from the American Type Culture Collection (ATCC). The HPV16-type SiHa, HPV18-type HeLa, and non-HPV-type Yumoto cell lines were maintained in Dulbecco’s Modified Eagle’s Medium (Life Technologies, CA, USA), supplemented with 10% fetal bovine serum. Cell lines were maintained in a humidified incubator containing 5% CO₂ at 37 °C. Cells were used for functional experiments within 3 months of passaging post-receipt. The SiHa, HeLa, and Yumoto cell lines were trypsinized and plated in culture dishes. At ~ 50% confluency, the cell lines were transfected with an annealed ADAR1 siRNA (siADAR1, sc-37657; Santa Cruz Biotechnology, TX, USA), control siRNA (siControl, sc-37007; Santa Cruz Biotechnology, TX, USA), or an empty vector (mock) for gene silencing (final concentration, 100 nmol/L) using an siRNA transfection reagent (sc-29528; Santa Cruz Biotechnology, TX, USA).