Anti mouse p53 cm5

Manufactured by Leica

Anti-mouse p53 (CM5) is a monoclonal antibody that specifically recognizes the p53 protein in mouse samples. It is a useful tool for the detection and analysis of p53 in immunohistochemistry and Western blot applications.

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Lab products found in correlation

Anti cleaved caspase 3, by Cell Signaling Technology (2 mentions) Anti puma, by Santa Cruz Biotechnology (2 mentions) Anti p21, by Santa Cruz Biotechnology (2 mentions) Anti wt1, by Santa Cruz Biotechnology (1 mentions) β actin, by Merck Group (1 mentions)

Close spelling variants of this product

Anti-p53 (14 citations) P53 (CM5; (6 citations)

3 protocols using anti mouse p53 cm5

Anemia and Tissue Analysis in Mice

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To determine if the mice were suffering anemia, mouse blood was collected from tail snip and a complete blood count was performed. For histopathological analysis, tissues were collected from the mice and processed according to standard procedures. For immunostaining, antibodies used in the study were: anti-mouse p53 (CM5, Novocastra), anti-cleaved Caspase3 (Cell Signaling 9661), p21 (Santa Cruz, SC397), ki67 (abcam, ab16667), and anti-WT1 (Wilms’ tumor antigen 1) (Santa Cruz, sc-192). The sections were counter stained with hematoxylin.

Kon N., Churchill M., Li H., Mukherjee S., Manfredi J.J, & Gu W. (2020). Robust p53 stabilization is dispensable for its activation and tumor suppressor function. Cancer research, 81(4), 935-944.

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Characterizing Hematopoietic Stem Cells

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Bone marrow cells were collected and stained with antibodies against c-kit and Sca-1. FACS analysis was gated on Lin^- cells and the cells positive for c-kit and Sca-1 were counted. For Western blot analysis, bone marrow cells were collected and the whole cell lysates were prepared with RIPA buffer. The proteins were separated by SDS-PAGE before transferred onto the membrane and probed with the indicated antibodies: anti-mouse p53 (CM5, Novocastra), anti-p21 (Santa Cruz, SX118), anti-PUMA (Santa Cruz, H-136). Levels of β-actin (Sigma) were used as total protein control.

Kon N., Churchill M., Li H., Mukherjee S., Manfredi J.J, & Gu W. (2020). Robust p53 stabilization is dispensable for its activation and tumor suppressor function. Cancer research, 81(4), 935-944.

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Analyzing Apoptosis Markers in Mouse Tissues

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The whole cell extracts were prepared from mouse tissues using RIPA buffer. The whole cell extracts of spleen or thymus from an untreated wild type mouse and an irradiated (6 Gy) wild type mouse were used as controls. The proteins were then resolved by SDS-PAGE, transferred onto the membrane, and probed using the following antibodies: anti-mouse p53 (CM5, Novocastra), anti-PUMA (Santa Cruz, H-136), anti-cleaved Caspase 3 (Cell Signaling 9661), anti-p21 (Santa Cruz Biotechnology, SX118). Levels of Vinculin were used as total protein control.

Kon N., Churchill M., Li H., Mukherjee S., Manfredi J.J, & Gu W. (2020). Robust p53 stabilization is dispensable for its activation and tumor suppressor function. Cancer research, 81(4), 935-944.

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