Tadalafil

Manufactured by Santa Cruz Biotechnology

Sourced in Spain, China, United States, United Kingdom

Tadalafil is a lab equipment product manufactured by Santa Cruz Biotechnology. It functions as a phosphodiesterase type 5 (PDE5) inhibitor.

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Lab products found in correlation

5 protocols using tadalafil

Pharmacological Evaluation of Tadalafil and Riociguat

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All drugs were from Sigma-Aldrich (Spain) except tadalafil (SantaCruz Biotechnology, USA) and riociguat (MedChem Express, China)

Chamorro V., Morales-Cano D., Milara J., Barreira B., Moreno L., Callejo M., Mondejar-Parreño G., Esquivel-Ruiz S., Cortijo J., Cogolludo Á., Barberá J.A, & Perez-Vizcaino F. (2018). Riociguat versus sildenafil on hypoxic pulmonary vasoconstriction and ventilation/perfusion matching. PLoS ONE, 13(1), e0191239.

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Mammalian Ringer Solution Protocol

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All the salts required to prepare the mammalian ringer solution were purchased from Fischer chemical limited, Guangzhou, China. Tadalafil (Code No: CAS-171596-29-5) was purchased from Santa Cruz Biotechnology, USA. All the fine chemicals used in the study were obtained from Qualigens, Mumbai, India. We used potassium chloride (KCl) to induce contraction in the ureteral tissue, as it has been used since a long time to artificially activate smooth muscle by a highly reproducible mechanism that works through the activation of calcium channels leading to increases in cytosolic-free Ca²⁺ and activation of myosin light-chain kinase followed by contraction.[10 (link)] KCl and Tadalafil were dissolved in double-distilled water or dimethyl sulfoxide and made into stock solutions.

Sakthivel M.S., Prabha R., Singh O., Kekre N.S, & Kumar S. (2018). The effect of phosphodiesterase-5 inhibitor, tadalafil, on in vitro potassium chloride-induced contractions of isolated human ureteral tissue. Indian Journal of Urology : IJU : Journal of the Urological Society of India, 34(4), 287-291.

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Preparation of Stock Solutions

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Potassium chloride (KCl; Qualigens, Mumbai, India), was dissolved in double-distilled water to obtain a concentration of 168 mg/ml. KCl was prepared fresh every day. Tadalafil (Santa Cruz Biotechnology, Dallas, TX, USA) was dissolved in DMSO (Sigma-Aldrich, St. Louis, MO, USA) to achieve a concentration of 5 mg/ml. Glibenclamide (Santa Cruz Biotechnology, Dallas, TX, USA) was dissolved in 90% ethanol to obtain a 2 mM stock solution. Iberiotoxin (Santa Cruz Biotechnology, Dallas, TX, USA) was dissolved in double-distilled water to obtain a 4 μM stock solution.

Sen S., Thomas A., Das S., Dey J.K., Peedicayil A., Thomas V, & Peedicayil J. (2016). Inhibition by tadalafil of contractility of isolated nonpregnant human myometrium. Journal of Pharmacology & Pharmacotherapeutics, 7(4), 177-181.

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Cardiomyocyte contractility regulation

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M199 medium (Invitrogen UK, 11150), taurine (Biochemica, A1141), creatine monohydrate (Sigma Aldrich, C3630), penicillin/streptomycin (Merck, A2212), carnitine hydrochloride (Sigma Aldrich, C9500) BSA (Sigma Aldrich, A6003), laminin (Sigma Aldrich L2020), isoproterenol hydrochloride (Sigma Aldrich, I6504), ICI118551 (Tocris UK, 0821), CGP 20712A (Tocris UK, 1024), SR 59230A hydrochloride (Tocris UK, 1511), L-NAME hydrochloride (Tocris UK, 0665), Vinpocetine (Sigma Aldrich, V6383), EHNA hydrochloride (Sigma Aldrich, E114), Cilostamide (Tocris UK, 0915), Tadalafil (Santa Cruz USA, sc-208412), IBMX (Santa Cruz sc-201188), self-made rabbit sGCα and β subunit antibodies (specificity tested in KO animals; Friebe et al., 2018 (link)), mouse α-actinin (Sigma Aldrich, A7732), mouse Caveolin-3 (BD Transduction Laboratories, 610421, specificity tested in KO animals; Woodman et al., 2002 (link)), secondary Alexa Fluor antibodies 488 nm, 514 nm, 568 nm and 633 nm (Life Technologies), BSA (Fisher Scientific UK, BPE9704), fluorescence mounting medium (Vectashield Germany, H-1000), MaTek glass-bottom dishes (MaTek USA, P35G-1.5–10 C), TAT-scram and TAT-Cav3 peptides (a gift from Dr. Sarah Calaghan from Leeds, England).

Schobesberger S., Wright P.T., Poulet C., Sanchez Alonso Mardones J.L., Mansfield C., Friebe A., Harding S.E., Balligand J.L., Nikolaev V.O, & Gorelik J. (2020). β3-Adrenoceptor redistribution impairs NO/cGMP/PDE2 signalling in failing cardiomyocytes. eLife, 9, e52221.

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Tadalafil Dosing in Mice

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Stock solutions of tadalafil (Santa Cruz Biotechnology, Santa Cruz, CA, SC-208412), were administered to pregnant and non-pregnant mice via oral gavage at 2 mg/kg body weight at one h post-exposure and every 24 h thereafter. Control mice received equal volumes of sterile saline by gavage.

Lambert J.A., Carlisle M.A., Lam A., Aggarwal S., Doran S., Ren C., Bradley W.E., Dell’Italia L., Ambalavanan N., Ford D.A., Patel R.P., Jilling T, & Matalon S. (2017). MECHANISMS AND TREATMENT OF HALOGEN INHALATION-INDUCED PULMONARY AND SYSTEMIC INJURIES IN PREGNANT MICE. Hypertension (Dallas, Tex. : 1979), 70(2), 390-400.

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