The HPLC gradient elution analysis was performed on Merck-Hitachi LaChrom Elite System (Merck, Darmstadt, Germany) with diode array detector L-2455, thermostat L-2300, pump L-2130 and autosampler L-2200 with the Kinetex RP18 (5 μm, 150 × 4.6 mm) chromatographic column (Phenomenex, Torrance, CA, USA) as stationary phase. The mobile phase was consisted of methanol-water-0.1% formic acid (gradient 5–100% (v/v) of methanol) at 30 °C and the run time was 60 min. The analysis was performed with the detection wavelength 280 nm, the sample injection volume was 10 μL and the flow rate was set to 1.0 mL min−1. Methanol Chromasolv for HPLC was purchased from Sigma-Aldrich (St. Louis, MO, USA) and formic acid from Polish Reagents (Polish Reagents, Gliwice, Poland); double-distilled water was used.
Methanol chromasolv for hplc
Manufactured by Merck Group
Sourced in United States
Methanol Chromasolv for HPLC is a high-purity solvent designed for use in high-performance liquid chromatography (HPLC) applications. It is a colorless, volatile liquid that meets the specifications required for HPLC-grade solvents.
Automatically generated - may contain errors
Lab products found in correlation
Lachrom elite system, by Merck Group (1 mentions)
Kinetex rp18, by Phenomenex (1 mentions)
Sodium hydroxide pellet, by Merck Group (1 mentions)
Polyvinyl alcohol (pva), by Merck Group (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Epigallocatechin, by Cayman Chemical (1 mentions)
Epicatechin gallate, by Cayman Chemical (1 mentions)
Epicatechin, by Cayman Chemical (1 mentions)
Catechin gallate, by Cayman Chemical (1 mentions)
Epigallocatechin gallate (egcg), by Cayman Chemical (1 mentions)
Apigenin, by Cayman Chemical (1 mentions)
Naringenin, by Cayman Chemical (1 mentions)
Quercetin, by Cayman Chemical (1 mentions)
Luteolin, by Cayman Chemical (1 mentions)
Gallic acid, by Cayman Chemical (1 mentions)
Diosmetin, by Extrasynthese (1 mentions)
Caffeine, by Merck Group (1 mentions)
Ringer solution, by Merck Group (1 mentions)
Phosphate buffer ph 6, by Merck Group (1 mentions)
4 protocols using methanol chromasolv for hplc
1
HPLC Gradient Elution Analysis of Compounds
2
Formulation and Characterization of AMX-Loaded PVA/PVP Hydrogels
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AMX in the form of AMX sodium was purchased from Haihang Industry Co. (Jinan, Shandong Province, China). Poly(vinyl alcohol) (PVA) (MW 85–124 kDa), ammonium acetate, acetic acid, sodium hydroxide pellets, acetonitrile for high performance liquid chromatography (HPLC) and methanol Chromasol V® for HPLC were all purchased from Sigma-Aldrich® (St Louis, USA). Poly(vinyl pyrrolidone) (PVP) (MW 58 kDa), sold under the product brand name Plasdone™ K-29/32, was a gift from Ashland Pharmaceuticals (Kidderminster, UK). Citric acid was purchased from BHD Laboratory Supplies (Poole, UK). Trifluoroacetic acid (TFA) was purchased from Tokyo Chemical Industry UK Ltd. (Oxford, UK). Sodium starch glycolate (SSG), sold under the product brand name Primojel®, was obtained from DFE Pharma (Klever Strasse, Germany). All other chemicals used were of analytical reagent grade.
3
Quantification of Phenolic Compounds
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The purity of all standards was >98%. Gallic acid, hydroxycinnamic acids (chlorogenic, caffeic, p-coumaric, and ferulic acids), the flavones luteolin, apigenin, luteolin-7-O-glucoside, and apigenin-7-O-glucoside, the flavonols quercetin (hydrate) and quercetin-3-O-rutinoside (rutin hydrate), the flavanones naringenin and diosmetin, the flavanols (±)-catechin (hydrate), (−)-epigallocatechin, (−)-epicatechin, and their gallate derivatives [(−)-catechin gallate, (−)-epicatechin gallate and (−)-epigallocatechin gallate], and the isoflavone daidzein were from Cayman Chemical Co. (Ann Arbor, MI, USA). The flavone diosmetin was from Extrasynthese Co. (Lyon, France). Methanol Chromasolv for HPLC was from Sigma Chemical (St. Louis, MO, USA).
4
Flufenamic Acid Sustained Release Formulation
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Materials used were: Hydroxyethyl cellulose (HEC) Natrosol® 250 M (Aqualon, Hercules Inc., Wilmington, DE, USA), Flufenamic acid, modification II (Kali-Chemie Pharma, Hannover, Germany), Poly(D,L-lactic-co-glycolic acid) (lactic to glycolic acid ratio 50:50) and a Mw distribution of 40,000–75,000 Da (Sigma Chemical Co., St. Louis, MO, USA), caffeine (Sigma Chemical Co., St. Louis, MO, USA), Polyvinyl alcohol (PVA) Mowiol® 4-88 (Kuraray Specialities Europe GmbH, Frankfurt, Germany). Ringer solution, McIlvaine citric acid phosphate buffer at different pH values, Phtalate buffer (pH 5.2), Phosphate buffer (pH 6) were obtained from Merck, Darmstadt, Germany as well as phosphoric acid and acetonitrile, n-octanol, propanol and potassium phosphate. Sodium hydroxide solution (0.1 M), Hydrochloric acid (0.1 M) was bought from Grüssing GmbH, Filsum, Germany. Methanol Chromasolv® for HPLC was from Sigma-Aldrich GmbH, Seelze, Germany. Ethyl acetate came from Fluka Chemie GmbH, Bucks, Switzerland. Cellulose membrane MWCO 12,000–14,000 Da for permeation experiments were acquired from Medicell International Ltd., London, LX, USA. Arabic gum (Caeser & Lorentz, Hilden, Germany), Gelatine A (Dt. Gelatine Fabriken, Eberbach, Germany), LysoSensor™ Green DND-189 (Invitrogen GmbH, Karlsruhe, Germany) were, as all other compounds, used as obtained from the suppliers.
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