Genomestudio genotyping analysis module v1

Example 3

The three DH populations, YSC, YDN, and TN, were genotyped with 12,000 SNP markers developed at DAS on two Illumina Infinium chips on the BeadStation 500 G per manufacturer's protocol (Illumina, San Diego, Calif.). Genotypic data was analyzed using the GenomeStudio Genotyping Analysis Module v1.8.4 (Illumina, San Diego, Calif.), which converts fluorescent signals for each SNP into A and B signals whose values reflect the relative abundance of arbitrarily assigned A and B alleles. Signal is converted into polar coordinates, using the Manhattan distance metric for the intensity R, and with Theta∈[0,1] representing angle ∈[0,90] degrees. Each marker is clustered in Cartesian coordinates, and the genotypes {AA, AB, BB} are assigned to samples in clusters close to Theta-{0, ½, 1}.

Example 3

The three DH populations, YSC, YDN, and TN, were genotyped with 12,000 SNP markers developed at DAS on two Illumina Infinium chips on the BeadStation 500 G per manufacturer's protocol (Illumina, San Diego, Calif.). Genotypic data was analyzed using the GenomeStudio Genotyping Analysis Module v1.8.4 (Illumina, San Diego, Calif.), which converts fluorescent signals for each SNP into A and B signals whose values reflect the relative abundance of arbitrarily assigned A and B alleles. Signal is converted into polar coordinates, using the Manhattan distance metric for the intensity R, and with Theta∈[0,1] representing angle∈[0,90] degrees. Each marker is clustered in Cartesian coordinates, and the genotypes {AA, AB, BB} are assigned to samples in clusters close to Theta={0, ½, 1}.