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Pe apoptosis detection kit 1

Manufactured by BD
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The PE Apoptosis Detection Kit is a laboratory tool designed to detect and analyze apoptosis, a type of programmed cell death, using flow cytometry. The kit provides the necessary reagents and protocols to quantify the percentage of cells undergoing apoptosis in a sample.

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Lab products found in correlation

Ezcount mtt cell assay kit, by HiMedia (1 mentions) Ldh activity, by Promega (1 mentions)

Close spelling variants of this product

PE Annexin V Apoptosis Detection Kit I (509 citations) Apoptosis detection kit (367 citations) Apoptosis Detection Kit I (66 citations) BD Pharmingen PE Annexin V Apoptosis Detection Kit I (12 citations) Annexin V-PE/7-AAD Apoptosis Detection Kit I (7 citations) Apoptosis kits (4 citations) Annexin V Phycoerythrin (PE) Apoptosis Detection Kit I (4 citations) PE-AnnexinV and 7-amino-actinomycin D (7-AAD) Apoptosis Detection Kit I (3 citations) PE Annexin V and 7-AAD Apoptosis Detection Kit I (2 citations) PE annexin apoptosis detection kit I (2 citations)

2 protocols using pe apoptosis detection kit 1

1

Determining TAK-242 Cytotoxicity in Macrophages

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The working concentrations of TAK-242 in different host macrophage systems were determined using either the AnnexinV-7-AAD-based method (Annexin V: PE Apoptosis detection kit I, catalog number: 559763; BD Biosciences, USA) or MTT assay-based method (EZcount™ MTT cell assay kit, catalog number: CCK-003-2500; HiMedia laboratories Pvt. Ltd, India) as per manufacturer’s protocol.
Mahish C., De S., Chatterjee S., Ghosh S., Keshry S.S., Mukherjee T., Khamaru S., Tung K.S., Subudhi B.B., Chattopadhyay S, & Chattopadhyay S. (2023). TLR4 is one of the receptors for Chikungunya virus envelope protein E2 and regulates virus induced pro-inflammatory responses in host macrophages. Frontiers in Immunology, 14, 1139808.
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2

Evaluating Cell Cytotoxicity and Apoptosis

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A total of 100 μl of the culture supernatant was removed from each well and added to a new plate to assay LDH activity (Promega) according to the manufacturer’s instructions. We further detected the rate of apoptosis using the PE Apoptosis Detection Kit I (BD) on the guava easyCyteTM HT system and analysis on guavaSoft software (Millpore).The MTT kit was used for the hepatocyte viability assay according to the manufacturer’s instructions (Life Technologies).
Wang H., Wang G., Zhang L., Zhang J., Zhang J., Wang Q, & Billiar T.R. (2016). ADAR1 Suppresses the Activation of Cytosolic RNA-Sensing Signaling Pathways to Protect the Liver from Ischemia/Reperfusion Injury. Scientific Reports, 6, 20248.
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