6410 triple quad lc ms mass spectrometer

ICR mice weighting 20 ± 2 g were divided into three groups at random (n = 12). Free ZL006, P-LPs/ZL006 and T7-P-LPs/ZL006 (all containing ZL006 4 mg/kg) were administered to each group through intravenous route, respectively. At designated time intervals (0.5, 1 and 2 h), the mice were executed and the major organs samples including brain, heart, liver, spleen, lung and kidney were collected. Before pretreatment, these tissues were rinsed with cold saline solution to remove the blood and then blotted with paper towel. Protein precipitation of the samples was performed before analysis31 (link). Then the samples were injected into the LC–MS/MS systems for analysis. The LC–MS/MS system consists of an Agilent Series 1200 HPLC system (Agilent Technologies, Waldbronn, Germany) and a 6410 Triple Quad LC/MS mass spectrometer (Agilent Technologies, Santa Clara, CA, USA). The data was collected and processed using the Agilent MassHunter Workstation Software (version B.01.04).

An Agilent Series 1200 HPLC system (Agilent Technologies, Waldbronn, Germany) and a 6410 Triple Quad LC/MS mass spectrometer (Agilent Technologies, Santa Clara, CA, USA) were used for the LC-MS/MS studies.
The liquid chromatography separations were performed on a Hypersil GOLD column (3 μm, 100 mm × 2.1 mm; Thermo Fisher Scientific, USA) at room temperature. The mobile phase consisted of solvent A (0.1% FA in water) and solvent B (0.1% FA in methanol). A linear gradient with a flow rate of 0.3 mL/min was applied in the following manner: B 10% (0 min) → 10% (1 min) → 90% (4 min) → 90% (8 min) → 10% (9 min). The injection volume was 10 μL.
The mass spectrometer was interfaced with an electrospray ion source and operated in the positive MRM mode. Q1 and Q3 were both set at unit resolution. The flow of the drying gas was 10 L/min, and the drying gas temperature was held at 350 °C. The electrospray capillary voltage was optimized to 4000 V. The nebulizer pressure was set to 45 psi. The data were collected and processed using the Agilent MassHunter Workstation Software (version B.01.04).