Cytokine levels in cell-free supernatants were assessed using standard sandwich ELISA for IL-5 and IL-13 (eBioscience) or using a commercially available ELISA kit for IL-9 (R & D Systems). For real-time PCR, RNA was isolated from lung tissue using an RNeasy Mini Kit (Qiagen, Valencia, CA) or from sort-purified cells using an RNeasy Micro Kit (Qiagen) according to the manufacturer instructions. RT-PCR or real-time PCR was performed on cDNA generated using a Superscript II reverse transcription kit (Life Technologies) using SYBR green master mix (Applied Biosystems) or Taqman Gene Expression Mastermix (Life Technologies) and commercially available primer sets (Qiagen Quantitect or Life Technologies Taqman primer assays). Samples were run on the ABI 7500 real-time PCR system (Life Technologies). RT-PCR products were run on a 3% agarose gel and visualized using a BioRad Gel Doc 1000 and Quantity One software.
Elisa kit for il 9
Manufactured by R&D Systems
The ELISA kit for IL-9 is a laboratory assay designed to quantitatively measure the concentration of interleukin-9 (IL-9) in biological samples. IL-9 is a cytokine that plays a role in various immunological processes. This kit provides the necessary reagents and protocols to perform the enzyme-linked immunosorbent assay (ELISA) technique for the detection and quantification of IL-9 levels.
Automatically generated - may contain errors
Lab products found in correlation
Gel doc 1000, by Bio-Rad (2 mentions)
Superscript 2 reverse transcription kit, by Thermo Fisher Scientific (2 mentions)
Standard sandwich elisa for il 5 and il 13, by Thermo Fisher Scientific (2 mentions)
Taqman gene expression master mix, by Thermo Fisher Scientific (2 mentions)
Sybr green master mix, by Thermo Fisher Scientific (2 mentions)
Abi 7500 real time pcr system, by Thermo Fisher Scientific (2 mentions)
Rneasy mini kit, by Qiagen (2 mentions)
Rneasy micro kit, by Qiagen (2 mentions)
2 protocols using elisa kit for il 9
1
Cytokine Detection in Lung Tissue
2
Cytokine Detection in Lung Tissue
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Cytokine levels in cell-free supernatants were assessed using standard sandwich ELISA for IL-5 and IL-13 (eBioscience) or using a commercially available ELISA kit for IL-9 (R & D Systems). For real-time PCR, RNA was isolated from lung tissue using an RNeasy Mini Kit (Qiagen, Valencia, CA) or from sort-purified cells using an RNeasy Micro Kit (Qiagen) according to the manufacturer instructions. RT-PCR or real-time PCR was performed on cDNA generated using a Superscript II reverse transcription kit (Life Technologies) using SYBR green master mix (Applied Biosystems) or Taqman Gene Expression Mastermix (Life Technologies) and commercially available primer sets (Qiagen Quantitect or Life Technologies Taqman primer assays). Samples were run on the ABI 7500 real-time PCR system (Life Technologies). RT-PCR products were run on a 3% agarose gel and visualized using a BioRad Gel Doc 1000 and Quantity One software.
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