Iron free ent

Manufactured by Merck Group

The Iron-free Ent is a laboratory equipment designed for the detection and analysis of enterobacteria. It is a selective and differential culture medium used to identify and distinguish between different types of Gram-negative bacteria, particularly members of the Enterobacteriaceae family. The core function of the Iron-free Ent is to facilitate the growth and identification of these microorganisms in a controlled laboratory setting.

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Lab products found in correlation

2 protocols using iron free ent

Human Lactoferrin Activation Assay

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Human MPO, iron-free Ent, ferric chloride, hydrogen peroxide, O-dianisidine hydrochloride, DHBA, ABAH, dimethyl sulfoxide, ferrichrome and 2,2^’ dipyridyl were procured from Sigma (St Louis, MO). Yersiniabactin (ybt) and salmochelin S4 were obtained from EMC microcollections (GmbH, Germany). Bovine milk LPO was purchased from Worthington Biochemical Corp., (Lakewood, NJ). Guaiacol (2-methoxyphenol) was obtained from Alfa Aesar (MA, USA). Human Lcn2 (also known as neutrophil gelatinase-associated lipocalin), murine Lcn2 (also known as 24p3) and Human MPO were purchased from R&D Systems (Minneapolis, MN). The commercially available recombinant Lcn2 proteins were free of endotoxin, siderophore and iron.

Singh V., Yeoh B.S., Xiao X., Kumar M., Bachman M., Borregaard N., Joe B, & Vijay-Kumar M. (2015). Interplay between enterobactin, myeloperoxidase and lipocalin 2 regulates E. coli survival in the inflamed gut. Nature communications, 6, 7113.

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Siderophore-Mediated Bacterial Interactions

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Iron-free Ent (E. coli), pyoverdine (Pseudomonas fluorescens), ferrichrome (Ustilago sphaerogena), deferoxamine mesylate salt, 2,3 dihydroxybenzoic acid (2,3-DHBA), PMA, LPS (E. coli 0128: B12), Ca⁺² ionophore (A23187), DMSO, ferric chloride, PIPES, Histopaque®-1077 and 1119, RPMI, LB, Dextran, BSA, PFA, Saponin, formyl-Met-Leu-Phe (fMLP) and kanamycin were procured from Sigma (St Louis, MO). Leukotriene B4 (LTB₄) was from Cayman Chemical (Ann Arbor, MI). Carrier-free mouse recombinant Lcn2 (free from endotoxin, siderophore, and iron) was obtained from Cell Signaling (Danvers, MA). Chrome azurol S (CAS) was purchased from Acros Organics (Geel, Belgium).

Saha P., Yeoh B.S., Olvera R.A., Xiao X., Singh V., Awasthi D., Subramanian B.C., Chen Q., Dikshit M., Wang Y., Parent C.A, & Vijay-Kumar M. (2017). Bacterial Siderophores Hijack Neutrophil Functions. Journal of immunology (Baltimore, Md. : 1950), 198(11), 4293-4303.

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