Ppk cd4 tdtom

Manufactured by Bloomington Drosophila Stock Center

Ppk-cd4-tdtom is a transgenic line that expresses the red fluorescent protein tdTomato under the control of the pickpocket (ppk) promoter, which drives expression in specific subsets of sensory neurons. This line allows for the visualization of these neurons in living tissue.

Automatically generated - may contain errors

Lab products found in correlation

Ppk gal4, by Bloomington Drosophila Stock Center (1 mentions) Uas lifeact rfp, by Bloomington Drosophila Stock Center (1 mentions) Ppk cd4 tdgfp, by Bloomington Drosophila Stock Center (1 mentions) Uas ivs gcamp6s, by Bloomington Drosophila Stock Center (1 mentions) Uas cd4 tdtom, by Bloomington Drosophila Stock Center (1 mentions) Uas yfp rab11, by Bloomington Drosophila Stock Center (1 mentions) Frt82b tubp gal80, by Bloomington Drosophila Stock Center (1 mentions)

2 protocols using ppk cd4 tdtom

Drosophila Genetic Toolbox for Mechanosensation

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All flies were maintained on standard medium at 23–25℃ in 12:12 light-dark cycles. 20×uas-ivs-gcamp6s (BL42746, BL42749), ppk-gal4 (BL32078, BL32079), uas-cd4-tdTom (BL35837, BL35841), ppk-cd4-tdtom (BL35845), ppk-cd4-tdgfp (BL35842, BL35843) uas-lifeact-rfp (BL58715), Ca-α1D^X10 (BL25141) and Mi{ET1}Ca-α1D^MB06807 (BL25527) were from Bloomington Drosophila Stock Center (BDSC). Gal4-19-12 was from the Jan Lab (UCSF). ppk26-gfp was from the Tracey Lab (Indiana University). Uas-mcherry-jupiter was from the Han Lab (Cornell University). Tmc-gal4, gfp-piezo, piezo^KO, uas-gfp-piezo, ppk26¹ and piezo^KO, ppk^Δ5 and were from Wei Zhang (Tsinghua University). tub-gal4 was from Bing Zhou (Tsinghua University). ctⁱ (THU1309), Ca-α1Dⁱ (THU0766) and the control strains were from Tsinghua Fly Center. Ca-α1D^AR66 was from Daniel Eberl (University of Iowa).

Liu Z., Wu M.H., Wang Q.X., Lin S.Z., Feng X.Q., Li B, & Liang X. (2022). Drosophila mechanical nociceptors preferentially sense localized poking. eLife, 11, e76574.

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Drosophila Rab11 Genetic Manipulation

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The Rab11^ΔFRT [26 (link)] was kindly provided by Dr. O. Schuldiner. spn-F² [30 (link)], ppk-eGFP [47 (link)], ppk-GAL4 [48 (link)] and UAS-IKKεIR [31 (link)] have been described previously. UAS-mCD8RFP was kindly provided by Dr. Y.N. Jan. The following stocks were obtained from Bloomington Stock Center: UAS-YFP-Rab11 (#9790); FRT82B, tubP-GAL80 (#5135); UAS-Luciferase-dsRNAs (#31603); ppk-CD4-tdTom (#35845); GSG2295 (#40266). The UAS-Rab11-dsRNAs (#108382) was obtained from the Vienna Drosophila RNAi Center (VDRC). The SOP-FLPs (#109944, #109945) were obtained from the Kyoto Stock Center. Flies were raised with regular fly foods at 25°C. Transgenic flies UAS-Rab11-GFP, UAS-Rab11-CA-GFP and UAS-Rab11-DN-GFP were generated by standard P-element mediated transformation.

Lin T., Kao H.H., Chou C.H., Chou C.Y., Liao Y.C, & Lee H.H. (2020). Rab11 activation by Ik2 kinase is required for dendrite pruning in Drosophila sensory neurons. PLoS Genetics, 16(2), e1008626.

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