Las x life science microscope software platform

Liver tissues were removed from the chick livers and fixed in 10% neutral formaldehyde. The samples were stained using Oil Red O, and the slices were observed through an optical microscope (DMi8; Leica, Germany) and photographed (LAS X Life Science Microscope Software Platform; Leica, Germany). In addition, cells were seeded in 12-well culture plates. After transfection and differentiation, the differentiated cells were washed in PBS and incubated with 10% neutral formaldehyde for 30 min. Subsequently, differentiated cells were dyed with Oil Red O, and observed with an optical microscope (DMi8; Leica, Germany) and photographed (LAS X Life Science Microscope Software Platform; Leica, Germany). Finally, the cell stain was extracted with isopropanol and analyzed at 510 nm absorbance with a Fluorescence/Multi-Detection Microplate Reader (BioTek, USA).

The coding sequences of FLS2, BIK1, and XLG2 were separately fused with nYFP, and the coding sequence of RipAW was fused with cYFP, as previously described [41 (link)]. The recombinant vector was verified by sequencing. The plasmids were transferred into A. tumefaciens GV3101 and infiltration of N. benthamiana was performed as described above. Infiltrated tissues were visualized to assess the interaction of FLS2-nYFP, BIK1-nYFP, or XLG2-nYFP with RipAW-cYFP at approximately 36 h after infiltration. Fluorescence was observed using a Leica TCS SP8 X White Light Laser Confocal Microscope (Leica, Wetzlar, Germany), and images were superimposed using the LAS X Life Science Microscope Software Platform (version 3.1.5.16308).