C57bl 6 il5tm1kopf j

Manufactured by Jackson ImmunoResearch

The C57BL/6-Il5tm1Kopf/J is a genetically modified mouse strain. It carries a targeted mutation in the interleukin 5 (Il5) gene, which is involved in the regulation of eosinophil production and function. This strain can be used for research purposes, but a detailed description of its core function is not available without the risk of bias or extrapolation.

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Lab products found in correlation

C b6 cg rag2tm1.1cgn j, by Jackson ImmunoResearch (1 mentions) Ob ob b6 cg lepob j, by Jackson ImmunoResearch (1 mentions) C57bl 6j, by Jackson ImmunoResearch (1 mentions)

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C57BL/6 (2462 citations)

2 protocols using c57bl 6 il5tm1kopf j

Comparative Analysis of Immune Responses in Genetically Modified Mice

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GITR deficient mice were obtained from Dr. Tania Watts (University of Toronto, Toronto, Canada) and Dr. Carlo Riccardi (University of Perugia, Perugia, Italy). C57BL/6J, Ob/Ob (B6.Cg-Lep^ob/J), RAG2 deficient (C.B6(Cg)-Rag2^tm1.1Cgn/J), IL-13 deficient (C.129P2-Il13^tm1.1Anjm) and IL-5 deficient (C57BL/6-Il5^tm1Kopf/J) mice were purchased from the Jackson Laboratory (Bar Harbor, Maine). All mice were bred in our animal facility at the Keck School of Medicine, University of Southern California (USC). Four to eight-week-old aged and sexed-matched mice were used in the studies. All animal studies were approved by the USC institutional Animal Care and Use Committee and conducted in accordance with the USC Department of Animal Resources’ guidelines.

Galle-Treger L., Sankaranarayanan I., Hurrell B.P., Howard E., Lo R., Maazi H., Lewis G., Banie H., Epstein A.L., Hu P., Rehan V.K., Gilliland F.D., Allayee H., Soroosh P., Sharpe A.H, & Akbari O. (2019). Costimulation of type-2 innate lymphoid cells by GITR promotes effector function and ameliorates type 2 diabetes. Nature Communications, 10, 713.

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Dietary n-3 PUFA Effects in Mice

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Male C57BL/6 or IL5^−/− (C57BL/6-Il5^tm1Kopf/J) mice, purchased from Jackson Laboratories, consumed a purified control low fat soybean oil enriched diet or a menhaden fish oil enriched diet (containing n-3 PUFAs) for 4 weeks, as previously described [12 (link)]. The n-3 PUFA enriched diet contained 1.3% of the total kcal from docosahexaenoic acid (DHA) and 2% from eicosapentaenoic acid (EPA). For a select study, C57BL/6 mice were fed high fat diets supplemented with EPA or DHA ethyl esters, as previously described [13 (link)]. Mice were sacrificed via CO₂ inhalation followed by cervical dislocation. All of the experiments with mice fulfilled the guidelines established by the East Carolina University Brody School of Medicine for euthanasia and humane treatment.

Teague H., Harris M., Whelan J., Comstock S.S., Fenton J.I, & Shaikh S.R. (2015). Short-term consumption of n-3 PUFAs increases murine IL-5 levels but IL-5 is not the mechanistic link between n-3 fatty acids and changes in B cell populations. The Journal of nutritional biochemistry, 28, 30-36.

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