Secondary antibodies of horseradish peroxidase conjugated goat anti rabbit igg

The following were used: total protein extraction kit and BCA protein quantization kits (Ken Gen Biotech. Co. Ltd, Nanjing, China), primary antibodies of TRAF6, NF-κB, PI3K, Akt, RANKL, OPG, β-actin and tubulin (Cell Signaling Technology, Beverly, MA, USA), secondary antibodies of horseradish peroxidase-conjugated goat anti-rabbit IgG (ZSGB-BIO, Beijing, China), estradiol valerate (1 mg, Delpharm Lille S.A.S, Paris, France), bone Gla-protein and deoxypyridinoline crosslinks ELISA kits (Xinyu Biological Engineering Co. Ltd., Shanghai, China) and the cathepsin K ELISA kit (Biovision, Mountain View, CA, USA). All other chemicals and biochemical agents used were of AR analytical grade reagent.

Acteoside was isolated from C. deserticola by using the above method (Figure 8, UHPLC ≥ 98%); RAW264.7 cells were obtained from Zhong Qiao Xin Zhou Biotechnology Co., Ltd. Shanghai, China; estradiol valerate was purchased from Delpharm Lille S.A.S, Paris, France; BGP, DPD, and TRAP crosslinks Elisa kits were bought from Xinyu Biological Engineering Co. Ltd., Shanghai, China; cathepsin K Elisa kit was purchased from Mountain View, CA, USA; MCSF and RANKL were provided by Pepro Tech Inc. American; total protein extraction kit and bicinchoninic acid (BCA) protein quantization kit were supplied by Ken Gen Biotech. Co. Ltd., Nanjing, China; primary antibodies including TRAF6, RANKL, RANK, NFKBIA, PI3K, AKT, IKKβ, NFAT2, c-Fos, β-actin, and secondary antibodies of horseradish peroxidase-conjugated goat anti-rabbit IgG were offered by ZSGB-BIO, Beijing, China; all other reagents were of analytical purity.