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Mouse il 4 ifn γ elisa kits

Manufactured by BioLegend

The Mouse IL-4/IFN-γ ELISA kits are sandwich enzyme-linked immunosorbent assays (ELISAs) designed to quantitatively measure mouse interleukin-4 (IL-4) and interferon-gamma (IFN-γ) levels in cell culture supernatants, serum, and plasma.

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2 protocols using mouse il 4 ifn γ elisa kits

1

Immunogenic Peptide Synthesis and Evaluation

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2-Cl-trityl chloride resin was obtained from Nankai Resin Co., Ltd. l- and d-Fmoc-amino acids and O-benzotriazole-N,N,N′,N′-tetramethyl-uronium-hexafluorophosphate (HBTU) were obtained from GL Biochem (Shanghai). 2-Naphthylacetic acid was obtained from Aladdin. Chemical reagents and solvents were used as received from commercial sources. Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS) and penicillin/streptomycin were purchased from Gibco Corporation. EndoFit ovalbumin (OVA) (endotoxins <1 EU mg−1) was purchased from InvivoGen (CA, USA). Horseradish peroxidase-conjugated goat anti-mouse IgG, IgG1, IgG2a and IgG2b were obtained from Southern Biotechnologies (AL, USA). Mouse IL-4/IFN-γ ELISA kits were purchased from Biolegend. Six-week-old female C57BL/6 mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd.
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2

Synthesis and Characterization of Immunogenic Peptide Conjugates

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2-Cl-trityl chloride resin was obtained from Nankai resin Co., Ltd. Fmoc-amino acids and O-Benzotriazole-N,N,N′,N′-tetramethyl-uronium-hexafluorophosphate (HBTU) were obtained from GL Biochem (Shanghai). Flurbiprofen was obtained from Aladdin. Chemical reagents and solvents were used as received from commercial sources. Fetal bovine serum (FBS), RPMI-1640 medium and penicillin/streptomycin were purchased from Gibco Corporation. EndoFit ovalbumin (OVA) (endotoxins <1 EU mg−1) was purchased from InvivoGen (CA, USA). Horseradish peroxidase-conjugated goat anti-mouse IgG, IgG1, IgG2a, and IgG2b were obtained from Southern Biotechnologies (AL, USA). Mouse IL-4/IFN-γ ELISA kits were purchased from Biolegend. Six-week-old female C57BL/6 mice were purchased from the Beijing Vital River Laboratory Animal Technology Co., Ltd.
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