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Goat α rat hrp

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Goat α-rat-HRP is a secondary antibody conjugated with horseradish peroxidase (HRP) that binds to rat primary antibodies. It is used in immunoassays and other applications where detection of rat antibodies is required.

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Lab products found in correlation

Rabbit α hltf a300 230a, by Fortis Life Sciences (2 mentions) Mouse α ung2, by OriGene (2 mentions) Goat α lamin b1 sc 6217, by Santa Cruz Biotechnology (2 mentions) Goat α mouse hrp, by Jackson ImmunoResearch (2 mentions) Goat α rabbit hrp, by Jackson ImmunoResearch (2 mentions) Rat α ha, by Roche (2 mentions) Pvdf membrane, by Merck Group (2 mentions) Chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions) Ibright cl1000 imaging system, by Thermo Fisher Scientific (2 mentions) Benzonase, by Merck Group (2 mentions)

2 protocols using goat α rat hrp

1

Western Blot Analysis of DNA Repair Proteins

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Cells were lysed in Laemmli buffer supplemented with 100 mM dithiothreitol (DTT) and benzonase (1:100, Sigma-Aldrich). Following denaturation at 65°C, samples were separated by SDS-PAGE and transferred to PVDF-membranes (Millipore) by TransBlot semi-dry transfer. Membranes were blocked in 5% (w/v) milk – PBS-T (0.2% Tween-20) and incubated with primary antibody in milk-PBS-T at 4°C overnight followed by incubation with HRP-conjugated secondary antibodies at room temperature (RT). Blots were developed using chemiluminescent substrates (Thermo Scientific) and visualised using either X-ray film or an iBright CL1000 imaging system (Thermo Fisher Scientific). The following antibodies were used for immunoblotting, listed by manufacturer: Primary antibodies: Abcam: Rabbit α-SLF2 (ab122480), rabbit α-VPRBP/DCAF1 (ab202587). Bethyl Laboratories: Rabbit α-HLTF (A300-230A). GeneTex: Rabbit α-SMC6 (GTX116832), rabbit α-NSMCE1 (GTX107136). NIH AIDS Reagent Program: Mouse α-Vif (#6459; (Simon et al., 1995 (link))). Origene: Mouse α-UNG2 (TA503563). Roche: Rat α-HA (11867423001). Santa Cruz: Goat α-Lamin B1 (sc-6217). Sigma-Aldrich: Mouse α-β-actin (A5316), rabbit α-ANKRD32/SLF1 (SAB2701555). Secondary antibodies: Jackson ImmunoResearch: Goat α-mouse-HRP (115-035-146), goat α-rabbit-HRP (115-035-144), and goat α-rat-HRP (115-035-143).
Dupont L., Bloor S., Williamson J.C., Cuesta S.M., Shah R., Teixeira-Silva A., Naamati A., Greenwood E.J., Sarafianos S.G., Matheson N.J, & Lehner P.J. (2021). The SMC5/6 complex compacts and silences unintegrated HIV-1 DNA and is antagonized by Vpr. Cell Host & Microbe, 29(5), 792-805.e6.
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2

Western Blotting of DNA Repair Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in Laemmli buffer supplemented with 100 mM dithiothreitol (DTT) and benzonase (1:100, Sigma-Aldrich). Following denaturation at 65°C, samples were separated by SDS-PAGE and transferred to PVDF-membranes (Millipore) by TransBlot semi-dry transfer. Membranes were blocked in 5% (w/v) milk – PBS-T (0.2% Tween-20) and incubated with primary antibody in milk-PBS-T at 4°C overnight followed by incubation with HRP-conjugated secondary antibodies at room temperature (RT). Blots were developed using chemiluminescent substrates (Thermo Scientific) and visualised using either X-ray film or an iBright CL1000 imaging system (Thermo Fisher Scientific). The following antibodies were used for immunoblotting, listed by manufacturer: Primary antibodies: Abcam: Rabbit α-SLF2 (ab122480), rabbit α-VPRBP/DCAF1 (ab202587). Bethyl Laboratories: Rabbit α-HLTF (A300-230A). GeneTex: Rabbit α-SMC6 (GTX116832), rabbit α-NSMCE1 (GTX107136). NIH AIDS Reagent Program: Mouse α-Vif (#6459; (Simon et al., 1995 (link))). Origene: Mouse α-UNG2 (TA503563). Roche: Rat α-HA (11867423001). Santa Cruz: Goat α-Lamin B1 (sc-6217). Sigma-Aldrich: Mouse α-β-actin (A5316), rabbit α-ANKRD32/SLF1 (SAB2701555). Secondary antibodies: Jackson ImmunoResearch: Goat α-mouse-HRP (115-035-146), goat α-rabbit-HRP (115-035-144), and goat α-rat-HRP (115-035-143).
Dupont L., Bloor S., Williamson J.C., Cuesta S.M., Shah R., Teixeira-Silva A., Naamati A., Greenwood E.J., Sarafianos S.G., Matheson N.J, & Lehner P.J. (2021). The SMC5/6 complex compacts and silences unintegrated HIV-1 DNA and is antagonized by Vpr. Cell Host & Microbe, 29(5), 792-805.e6.
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