Cxcr5 pe

Manufactured by R&D Systems

CXCR5 PE is a laboratory reagent used for the detection and analysis of the CXCR5 receptor in flow cytometry applications. CXCR5 is a chemokine receptor that plays a role in the migration and positioning of B cells. The CXCR5 PE reagent can be used to identify and quantify CXCR5-expressing cells in various sample types.

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Lab products found in correlation

Cd8 apc, by Thermo Fisher Scientific (1 mentions) Pd 1 pe, by BD (1 mentions) Icos pe cy7, by Thermo Fisher Scientific (1 mentions) Ccr6 pe, by BD (1 mentions) Cd4 fitc, by Beckman Coulter (1 mentions) Cd4 apc cy7, by Thermo Fisher Scientific (1 mentions) Icos pe, by BioLegend (1 mentions) Pd1 pe vio770, by Miltenyi Biotec (1 mentions) Cd45ra pe cy5, by BioLegend (1 mentions) Attune flow cytometry, by Thermo Fisher Scientific (1 mentions) Lsr 2 flow cytometer, by BD (1 mentions) Cd10 pecf594, by BD (1 mentions) Cd38 fitc, by BD (1 mentions) Igd pe cy7, by BioLegend (1 mentions) Cd40 apc, by BD (1 mentions) Cd4 pacblue, by BioLegend (1 mentions) Igm percp cy5, by BioLegend (1 mentions) Cd45ra ecd, by Beckman Coulter (1 mentions) Cd3 af700, by BioLegend (1 mentions) Cd21 pe, by BD (1 mentions)

2 protocols using cxcr5 pe

Immunophenotyping of T Cell Subsets

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PBMCs were separated by a gradient centrifugation procedure on a lymphocyte separation medium (Secoll Separation Media, Mannheim, Germany). After separation, freshly isolated cells were stained with the following fluorophore-conjugated antibodies: CD4 APC-Cy7 (clone: RFT-4g), ICOS PE-Cy7 (clone: ISA-3, eBioscience, ThermoFisher), CXCR5 FITC (CD185, clone: REA303), CXCR3 APC (CD183, clone: REA232), PD-1 PE-Vio 770 (clone: PD-1.3.1.3, Miltenyi Biotec), CD45RA PE-Cy5 (clone: HI100, BioLegend), and CCR6 PE (CD196, clone: 11A9, BD Bioscience).
The freshly isolated thymocytes and the PBMCs obtained at the time of thymectomy were stained with the following fluorochrome-conjugated antibodies: CD4 FITC (clone: RPA-T4, Beckman Coulter), CD8 APC (clone: RPA-T8), CXCR3 eFluor660 (CD183, clone: CEW33D, eBioscience), CCR6 PE (CD196, clone: 11A9), PD-1 PE (CD279, clone: MIH4, BD Bioscience), ICOS PE (clone: C398.4A, BioLegend), and CXCR5 PE (CD185, clone: 51505, R&D Systems). The samples were analyzed on an Attune Flow Cytometry (Thermofisher, USA). Fluorescence minus one control, which contains all flurochromes in a panel except for the target markers measured, was used to identify and to gate the cells.

Çebi M., Durmus H., Aysal F., Özkan B., Gül G.E., Çakar A., Hocaoglu M., Mercan M., Yentür S.P., Tütüncü M., Yayla V., Akan O., Dogan Ö., Parman Y, & Saruhan-Direskeneli G. (2020). CD4+ T Cells of Myasthenia Gravis Patients Are Characterized by Increased IL-21, IL-4, and IL-17A Productions and Higher Presence of PD-1 and ICOS. Frontiers in Immunology, 11, 809.

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Comprehensive Immune Cell Phenotyping

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PBMC (1–4×10⁶ cells/tube) were stained with monoclonal antibodies to B cell markers (CD19-AF700, IgM-PerCP-Cy5.5, IgD-PE-Cy7 (Biolegend), CD38-FITC, CD21-PE, CD10-PE-CF594, CD40-APC, CD86-BV421 (BD Pharmingen)) and T cell markers (CD3-AF700, CD4PacBlue (Biolegend), CD8-APC-AF750 (Invitrogen), CD45RA-ECD, CD27-PE-Cy5 (Beckman Coulter), CD27-BV650, CD38-FITC, HLA-DR-PE-Cy7, PD-1-APC, CXCR5-PE (R&D Systems)) for 40 minutes at room temperature. Stained cells were subsequently washed twice and fixed in 1% paraformaldehyde for 10 minutes at 4°C. Data was acquired within 4 hours using a BD LSRII flow cytometer (BD Biosciences – Immunocytometry Systems, San Jose, CA). Data were analyzed using Flow Jo Software (Tree Star Inc., Ashland, OR) with the gating scheme as per Supplemental Figure 1.

Nicholson L.K., Pratap H., Bowers E., Gunzburger E., Bandi S., Gardner E.M., Palmer B.E., Wright T., Kittelson J, & Janoff E.N. (2018). Effective B cell Activation in vitro during Viremic HIV-1 Infection with Surrogate T cell Stimulation. Immunobiology, 223(12), 839-849.

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