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2 protocols using standard lycorine

1

Evaluation of Lycorine's Anti-Inflammatory Potential

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Standard lycorine (purity > 98%) was purchased from Chem Faces (Wuhan, China). Dimethyl sulfoxide (DMSO), phosphoric acid, triethylamine, and purified water were prepared by Milli Q® system from Millipore (Bedford, MA, USA). Acetonitrile HPLC grade was purchased from RCI Lab Scan (Bangkok, Thailand). Murine macrophage leukemia cell line (RAW 264.7: ATCC® TIB-71™) was purchased from American Type Culture Collection (ATCC®, VA, USA). Fetal bovine serum (FBS) was purchased from Gibco® (OK, USA). Dulbecco's Modified Eagle's Medium (DMEM), phosphate buffer saline (PBS), and penicillin-streptomycin (P/S) were purchased from Biochrom (MA, Germany). 3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) was purchased from Sigma (MO, USA).
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2

Isolation and Evaluation of C. asiaticum Leaves

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C. asiaticum L. leaves, specimen voucher (SKP-008 03 01 01) was collected from the Faculty of Medicine, Thammasat University, Pathumthani, Thailand. The specimen voucher was deposited at the herbarium of the Southern Center of Thai Medicinal Plants, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Thailand. Carrageenan and Locust bean gum were purchased from Beauty Cosmetic Co., Ltd., Korea. Glycerin was purchased from Sigma-Aldrich, USA. Standard lycorine (purity > 98%) was purchased from Chem Faces (Wuhan, China). Triethylamine, phosphoric acid, dimethyl sulfoxide (DMSO), and purified water were prepared by Milli Q® system from Millipore (Bedford, MA, USA). Acetonitrile high-performance liquid chromatography (HPLC) grade was purchased from RCI LabScan (Bangkok, Thailand). 3-(4, 5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) was purchased from Sigma (MO, USA). The murine macrophage leukemia cell line (RAW 264.7: ATCC® TIB-71™) was purchased from American Type Culture Collection (ATCC®, VA, USA).
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