Axioimager z1 light microscope

Manufactured by Zeiss

Sourced in Germany

The Zeiss AxioImager Z1 is a light microscope designed for high-performance imaging. It features a range of advanced optical components and a modular design to support a variety of imaging techniques.

Automatically generated - may contain errors

Lab products found in correlation

Axiocam digital camera, by Zeiss (2 mentions) Mrc5 camera, by Zeiss (1 mentions) Jem 1200ex electron microscope, by JEOL (1 mentions) Tecnai g2 spiritbt, by Thermo Fisher Scientific (1 mentions) Axiovision software 4, by Zeiss (1 mentions) Axiocam mrc5 camera, by Zeiss (1 mentions) Axiocam mrm camera, by Zeiss (1 mentions) Hematoxylin and eosin, by Bio-Optica (1 mentions) Surgipath decalcifier, by Leica (1 mentions) Paraffin, by Bio-Optica (1 mentions)

7 protocols using axioimager z1 light microscope

Perifoveal and Peripheral Retina Histology

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fragments of retina–RPE–choroid were cut from the perifovea and periphery. Tissue fragments were further fixed by immersion in 2.5% GA in 0.1 M cacodylate buffer, post-fixed with 1% osmium tetroxide for 45 min on ice, sequentially dehydrated in ethanol, and embedded in Epon as previously described (Bonilha et al., 2015 (link)). Toluidine blue-stained sections were photographed with a Zeiss AxioImager. Z1 light microscope equipped with an MRc5 camera (Carl Zeiss AG, Oberkochen, Germany).

Bonilha V.L., Bell B.A., DeBenedictis M.J., Hagstrom S.A., Fishman G.A, & Hollyfield J.G. (2020). Cellular Changes in Retinas From Patients With BEST1 Mutations. Frontiers in Cell and Developmental Biology, 8, 573330.

+ Open protocol

+ Expand

Retinal Tissue Sampling and Ultrastructural Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Isolated fragments of retina-RPE-choroid in the edge of the GA lesion were cut from the following regions: superior (region 1), temporal (region 2), inferior (region 3), and nasal (region 4) as shown in Figure 2A (white rectangles). Tissue was further fixed by immersion in 2.5% glutaraldehyde in 0.1M cacodylate buffer, post-fixed with 1% osmium tetroxide for 45 minutes on ice, sequentially dehydrated in ethanol, and embedded in Epon as previously described.²⁰ (link) Toluidine blue–stained sections were photographed with a Zeiss AxioImager.Z1 light microscope using a ×20, numerical aperture 0.5 plan neofluar objective (Zeiss, 440340) and analyzed for occurring frequencies of existence of RPE cells within 400 µm of the edge of the GA lesion. Statistical analysis was performed as described below. Ultrathin sections were prepared, and electron micrographs were taken on a Tecnai G2 SpiritBT operated at 80 kV (FEI Company, Hillsboro, OR, USA) and on a JEM 1200-EX electron microscope (JEOL, Peabody, MA, USA).

Bonilha V.L., Bell B.A., Hu J., Milliner C., Pauer G.J., Hagstrom S.A., Radu R.A, & Hollyfield J.G. (2020). Geographic Atrophy: Confocal Scanning Laser Ophthalmoscopy, Histology, and Inflammation in the Region of Expanding Lesions. Investigative Ophthalmology & Visual Science, 61(8), 15.

+ Open protocol

+ Expand

Cre-Lox Genetic Labeling in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Heterozygous CAGGCre-ER TM , α-Cre or Lmop-Cre mice were crossed with homozygous Cre-reporter (R26R) mice (genetic background: 129SV) [19] (link). The resulting CAGGCre-ER TM (or α-Cre or Lmop-Cre)/R26R mice were used as experimental mice, and R26R littermates as control mice. Lac-Z-staining was performed in mixed CAGGCre-ER TM (or α-Cre or Lmop-Cre)/R26R and R26R mice following a previously published protocol [18] (link). Paraffin sections (6 µm thick) were analyzed using an Axio Imager Z1 light microscope (Carl Zeiss Microscopy GmbH, Jena, Germany) and the appropriate Axiovision software 4.8 (Carl Zeiss Microscopy GmbH, Jena, Germany).

Boneva S.K., Groß T.R., Schlecht A., Schmitt S.I., Sippl C., Jägle H., Volz C., Neueder A., Tamm E.R, & Braunger B.M. (2016). Cre recombinase expression or topical tamoxifen treatment do not affect retinal structure and function, neuronal vulnerability or glial reactivity in the mouse eye. Neuroscience, 325.

+ Open protocol

+ Expand

Visualization of F2rl1 Expression in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Pregnant female mice from breeding pairs heterozygous β-galactosidase-tagged F2rl1 knock-in mice were euthanized at E12.5 by cervical dislocation. Embryos were extracted by Caesarian section, and the individual embryos and placentas were dissected and placed in 2% PFA with 0.2% glutaraldehyde. Fixed tissues were stained overnight at room temperature in 1 mg/ml X-gal, 5 mM potassium ferricyanide, 5 mM potassium ferrocyanide, 2 mM magnesium chloride and 0.02% NP-40 in PBS. The tissues were post-fixed for 16 h in 4% PFA, embedded in paraffin, and sectioned. The sections were counterstained with nuclear fast red. All microscopic images were acquired on a Zeiss AxioImager Z1 light microscope (Carl Zeiss AG, Gottingen, Germany) using a Qicam FAST1394 digital camera from Qimaging.

Szabo R., Peters D.E., Kosa P., Camerer E, & Bugge T.H. (2014). Regulation of Feto-Maternal Barrier by Matriptase- and PAR-2-Mediated Signaling Is Required for Placental Morphogenesis and Mouse Embryonic Survival. PLoS Genetics, 10(7), e1004470.

+ Open protocol

+ Expand

Retinal Tissue Preparation for Microscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols

Eyes were enucleated and fixed by immersion in 2% paraformaldehyde, 2.5% glutaraldehyde and 5% CaCl₂ made in 0.1 M cacodylate buffer for 30 min at 4°C. Eyecups from both control and DJ-1 KO littermates were post-fixed in 1% osmium tetroxide, dehydrated in a graded series of ethanol to propylene oxide, embedded in epon and polymerized for 48 hrs at 60°C (Bonilha et al., 2006 (link)). For bright-field microscopy, semi-thin sections were cut with a diamond histotech knife, collected on glass slides, and stained with toluidine blue. Slides were photographed with a Zeiss AxioImager.Z1 light microscope, and the images were digitized using a Zeiss AxioCam MRc5 camera. For transmission electron microscopy (TEM), the same block of plastic-embedded samples was thin sectioned on an RMC, PowerTome-XL (Tucson, AZ) ultramicrotome, stained with uranyl acetate and lead citrate and viewed in a Tecnai 20–200 kV digital electron microscope (Philips, Hillsboro, OR) equipped with a Gatan image filter and digital camera at 3600 diameters as previously described (Bonilha et al., 2006 (link)).

Bonilha V.L., Bell B.A., Rayborn M.E., Yang X., Kaul C., Grossman G.H., Samuels I.S., Hollyfield J.G., Xie C., Cai H, & Shadrach K.G. (2015). Loss of DJ-1 elicits retinal abnormalities, visual dysfunction, and increased oxidative stress in mice. Experimental eye research, 139, 22-36.

+ Open protocol

+ Expand

Time-lapse Imaging of OYT1 and R-1

Check if the same lab product or an alternative is used in the 5 most similar protocols

Time-lapse experiments of OYT1 and R-1 were performed in microslide chambers as previously described by Krepski et al. (2013) (link) with minor modifications. The modified Wolfe’s minimal medium (MWMM) buffered with MES (adjusted to pH 6.2) was used as a basal medium as previously described (Kato et al., 2014 (link)). An FeS plug (without agar) was used as an iron source. Differential interference contrast images were captured every 5 min at 400x total magnification using a Zeiss AxioImager Z1 light microscope (Zeiss, Oberkochen, Germany) equipped with a Zeiss Axiocam Mrm camera. A Zeiss AxioVision software was used for image processing.

Kato S., Ohkuma M., Powell D.H., Krepski S.T., Oshima K., Hattori M., Shapiro N., Woyke T, & Chan C.S. (2015). Comparative Genomic Insights into Ecophysiology of Neutrophilic, Microaerophilic Iron Oxidizing Bacteria. Frontiers in Microbiology, 6, 1265.

+ Open protocol

+ Expand

Histological Assessment of Zebrafish and Medaka

Check if the same lab product or an alternative is used in the 5 most similar protocols

Eight samples for histological assessment were collected from control and each treatment at 5 dpf for zebrafish and at 14 dpf for medaka. The larvae were anesthetized with 0.01% MS 222 (Tricaine Methanesulfonate), and then immediately fixed in the Surgipath Decalcifier (Leica) for 24 h at 4°C. After dehydration in ethan ol, all specimens were embedded in paraffin (Bio-Optica, Italy) and sectioned at a thickness of 5 µm with a rotary automatic
microtome (Leica Microsystems, Wetzlar, Germany). Serial sections were stained with hematoxylin and eosin (Bio-Optica, Isttaly), and examined with a motorized Zeiss Axio Imager Z1 light microscope (Carl Zeiss AG, Werk Göttingen, Germany), equipped with an AxioCam digital camera (Zeiss, Jena, Germany) for the acquisition of images. This protocol was already published (Fasulo et al. 2010 , Maisano et al. 2016 , Maisano et al. 2017)

Vignet C., Cappello T., Fu Q., Lajoie K., De Marco G., Clérandeau C., Mottaz H., Maisano M., Hollender J., Schirmer K, & Cachot J. (2019). Imidacloprid induces adverse effects on fish early life stages that are more severe in Japanese medaka (Oryzias latipes) than in zebrafish (Danio rerio). Chemosphere, 225.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!