Liver caspase activity was measured as described (Lawson et al. 1999 (link)). In brief, frozen liver tissue was homogenized in 25 mM HEPES buffer containing 5 mM EDTA, 2 mM DTT and 0.1% CHAPS, and then centrifuged to get the homogenate. A fluorogenic substrate (Ac-DEVD-AFC, Enzo Life Sciences, Plymouth Meeting, PA) was added to the homogenate and fluorescence was measured with or without the presence of pan-caspase inhibitor (z-VAD-fmk, Enzo). Results are expressed as RFU per unit time per mg protein concentration. Western blotting was performed as described (Bajt et al. 2000 (link)) using a rabbit anti-caspase 3 antibody and a rabbit anti-beta-actin antibody (Cell Signaling Technology, Danvers, MA), and an anti-RIP3 antibody (Santa Cruz Biotechnology, Santa Cruz, CA). The proteins were visualized using a goat anti-rabbit HRP conjugated antibody (Santa Cruz Biotechnology Santa Cruz, CA).
Anti rip3 antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United Kingdom
The Anti-RIP3 antibody is a tool used in research applications. It is designed to detect the expression of the RIP3 protein, which plays a role in programmed cell death pathways. The antibody can be used in various experimental techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of RIP3 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Hrp conjugated goat anti rabbit antibody, by Santa Cruz Biotechnology (1 mentions)
Ac devd afc, by Enzo Life Sciences (1 mentions)
Rabbit anti beta actin antibody, by Cell Signaling Technology (1 mentions)
Z vad fmk, by Enzo Life Sciences (1 mentions)
Anti camkii antibody, by Abcam (1 mentions)
Kn 93, by Selleck Chemicals (1 mentions)
Cholecystokinin cck, by Merck Group (1 mentions)
Anti gapdh antibody, by Merck Group (1 mentions)
Anti rip3 antibody, by Abcam (1 mentions)
Caerulein, by Merck Group (1 mentions)
Anti pmlkl antibody, by Abcam (1 mentions)
Anti cd68 antibody, by Abcam (1 mentions)
Anti myeloperoxidase mpo, by Abcam (1 mentions)
Anti rabbit and anti mouse secondary antibody, by Cell Signaling Technology (1 mentions)
Ldh cytotoxicity assay kit, by Beyotime (1 mentions)
Hoechst 33342, by Keygen Biotech (1 mentions)
Lipase kits, by Nanjing Jiancheng (1 mentions)
2 protocols using anti rip3 antibody
1
Liver Caspase Activity Assay
2
Evaluation of Pancreatitis-related Markers
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KN93 was obtained from Selleck (Selleckchem, Texas, USA); caerulein, cholecystokinin (CCK), and DHE were purchased from Sigma (Sigma-Aldrich, St. Louis, MO, USA); anti-CaMK II antibody, anti-p-CaMK II antibody, anti-RIP3 antibody, anti-pMLKL antibody, anti-CD68 antibody, and anti-myeloperoxidase (MPO) were purchased from Abcam (Cambridge, UK); anti-GAPDH antibody was purchased from Sigma; and anti-RIP3 antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The anti-rabbit and anti-mouse secondary antibodies were purchased from Cell Signaling Technologies (Beverly, MA, USA). The LDH Cytotoxicity Assay Kit was purchased from Beyotime Biotechnology (Beijing, China); amylase kits were purchased from BioSino (BioSino BioTechnology & Science Inc., Beijing, China), and lipase kits were purchased from Nanjing Jiancheng (Nanjing Jiancheng Corp, Nanjing, China). Hoechst 33342 and propidium iodide (PI) were purchased from KeyGEN BioTECH, Nanjing, China. All flow cytometry-related antibodies were purchased from BioLegend.
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