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Total cholesterol

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The Total Cholesterol assay is a laboratory test used to measure the total amount of cholesterol present in a blood sample. It provides a comprehensive assessment of an individual's cholesterol levels, which is an important indicator of cardiovascular health.

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Lab products found in correlation

Free and total glycerol, by Merck Group (2 mentions) Monoclonal mouse anti glucagon antibody, by Merck Group (2 mentions) Insulin rabbit mab, by Cell Signaling Technology (2 mentions) Interleukin 6 (il 6), by R&D Systems (1 mentions) Insulin, by Crystal Chem (1 mentions) Enzymatic assay kit, by Thermo Fisher Scientific (1 mentions) Non esterified fatty acid, by Fujifilm (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Collagenase type xi, by Merck Group (1 mentions) Cmrl 1066, by Thermo Fisher Scientific (1 mentions) Immunocruz rabbit abc staining system, by Santa Cruz Biotechnology (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Cleaved caspase 3 rabbit mab, by Cell Signaling Technology (1 mentions) Hank s balanced salt solution, by Merck Group (1 mentions) Periodic acid, by Thermo Fisher Scientific (1 mentions) Schiff reagent, by Thermo Fisher Scientific (1 mentions) Alexa 488 conjugated donkey anti mouse, by Jackson ImmunoResearch (1 mentions)

Spelling variants of this product

Cholesterol (73 citations) Infinity Total Cholesterol Reagent (11 citations) Total cholesterol reagent (5 citations) Infinity Total Cholesterol (4 citations) Plasma total cholesterol (2 citations) Total Cholesterol Reagents #TR13421 (2 citations)

9 protocols using total cholesterol

1

Plasma Metabolite Quantification Protocol

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Plasma insulin levels were determined using an ELISA kit (Crystal Chem, Downers Grove, IL). Plasma levels of total cholesterol (Thermo Electron, Louisville, CO) and free and total glycerol (Sigma) were determined using commercial colorimetric assays. Plasma true triglyceride concentrations were estimated by subtraction of free glycerol from total glycerol.
Parkman J.K., Denvir J., Mao X., Dillon K.D., Romero S., Saxton A.M, & Kim J.H. (2017). Congenic Mice Demonstrate The Presence of QTLs Conferring Obesity and Hypercholesterolemia on Chromosome 1 in the TALLYHO Mouse. Mammalian genome : official journal of the International Mammalian Genome Society, 28(11-12), 487-497.
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2

Plasma Lipid and Cytokine Analysis

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Blood was collected when animals were euthanized at 17-18 weeks of age and plasma obtained by centrifugation (1,200g) at 4°C. Plasma levels of total cholesterol (Thermo Electron, Louisville, CO) and free and total glycerol (Sigma, St. Louis, MO) were determined using colorimetric assays. Plasma true triglyceride concentrations were estimated by subtraction of free glycerol from total glycerol. Plasma IL6 and Insulin levels were determined using an ELISA kit according to manufacturer’s instructions (IL6, R&D System, Minneapolis, MN; Insulin, Crystal Chem, Downers Grove, Illinois).
Parkman J.K., Sklioutovskaya-Lopez K., Menikdiwela K.R., Freeman L., Moustaid-Moussa N, & Kim J.H. (2021). Effects of high fat diets and supplemental tart cherry and fish oil on obesity and type 2 diabetes in male and female C57BL/6J and TALLYHO/Jng mice.. The Journal of nutritional biochemistry, 94, 108644.
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3

Multiparametric Metabolic Profiling

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Plasma glucose, alanine amino transferase (ALT), uric acid, total cholesterol, HDL cholesterol (Thermo Electron, Pittsburgh, PA), and NEFA (WAKO Pure Chemical Industries Ltd., Osaka, Japan) were determined using a Konelab 20X (Thermoscientific, Vantaa, Finland). Plasma leptin and insulin concentrations were measured using commercially available immunoassay kits (Crystal Chem Inc, Downers Grove, IL, USA and ALPCO Diagnostics, Salem, NH, USA).
All assays were conducted in accordance with the manufacturer's instructions and intra-and inter-assay coefficients of variation were always <10%.
Gugusheff J., Sim P., Kheng A., Gentili S., Al-Nussairawi M., Brand-Miller J, & Muhlhausler B. (2016). The effect of maternal and post-weaning low and high glycaemic index diets on glucose tolerance, fat deposition and hepatic function in rat offspring. Journal of developmental origins of health and disease, 7(3).
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4

Enzymatic Assay for Lipid Profiling

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Enzymatic assay kits were used to measure total cholesterol (Thermo Fisher, Ottawa, ON, Canada), unesterified cholesterol, and HDL-cholesterol (FujiFilm Medical Systems, formerly Wako Diagnostics, Richmond, VA) following the manufacturer’s instructions. Cholesteryl ester and non-HDL cholesterol values were determined by subtraction of unesterified cholesterol or HDL-cholesterol (respectively) from total cholesterol.
Kluck G.E., Qian A.S., Sakarya E.H., Quach H., Deng Y.D, & Trigatti B.L. (2022). Apolipoprotein A1 Protects Against Necrotic Core Development in Atherosclerotic Plaques: PDZK1-Dependent High-Density Lipoprotein Suppression of Necroptosis in Macrophages. Arteriosclerosis, Thrombosis, and Vascular Biology, 43(1), 45-63.
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5

Plasma Biomarker Measurement Protocol

Cited 3 times
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Plasma levels of alanine transaminase (ALT) and aspartate transaminase (AST) were determined using kinetic absorbance assays (Teco Diagnostics) as described previously [16 (link)]. Analysis of plasma triglycerides (Thermo Fisher Scientific), total cholesterol (Thermo Fisher Scientific), and non-esterified fatty acids (FUJIFILM Wako) were determined using colorimetric based assay as previously reported [50 (link), 51 (link)].
Habibi M., Ferguson D., Eichler S.J., Chan M.M., LaPoint A., Shew T.M., He M., Lutkewitte A.J., Schilling J.D., Cho K.Y., Patti G.J, & Finck B.N. (2023). Mitochondrial Pyruvate Carrier Inhibition Attenuates Hepatic Stellate Cell Activation and Liver Injury in a Mouse Model of Metabolic Dysfunction-associated Steatotic Liver Disease. bioRxiv.
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6

Comprehensive Metabolic Biomarker Assessment

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Immunoassays were used to measure total GIP (Millipore, ELISA), total GLP-1, and insulin (Meso Scale Discovery). Enzymatic assays were used to measure TG, beta-hydroxybutarate (Sigma-Aldrich), and total cholesterol (Thermo Fisher). HDL-cholesterol was determined in the supernatant after removal of LDL/vLDL-cholesterol by a precipitation reagent (Sigma-Aldrich); non-HDL-cholesterol was determined by subtracting HDL from total cholesterol. Free fatty acids were measured by GC-MS.
Lin H.V., Efanov A.M., Fang X., Beavers L.S., Wang X., Wang J., Gonzalez Valcarcel I.C, & Ma T. (2016). GPR142 Controls Tryptophan-Induced Insulin and Incretin Hormone Secretion to Improve Glucose Metabolism. PLoS ONE, 11(6), e0157298.
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7

Lipid Quantification in Liver Samples

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Liver samples (0.1 g) were homogenized in 2 ml Folch (chloroform/methanol, 2:1, v/v) with a polytron homogenizer. The organic phase was separated and then dried under nitrogen. Samples were reconstituted in isopropranol:Triton-X100 (9:1 v/v) and aliquots subjected to colorimetric enzymatic assays for total cholesterol (Thermo Scientific) or triglycerides (Pointe Scientific).
Adamson S.E., Polanowska-Grabowska R., Marqueen K., Griffiths R., Angdisen J., Breevoort S.R., Schulman I.G, & Leitinger N. (2018). Deficiency of Dab2 in myeloid cells exacerbates inflammation in liver and atherosclerotic plaques in LDL-R null mice. Arteriosclerosis, thrombosis, and vascular biology, 38(5), 1020-1029.
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8

Synthesis and Characterization of SR-135 Compounds

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SR-135 and its control compound SRB were synthesized as previously reported (31 (link)). Collagenase type XI, Hanks’ balanced salt solution, and monoclonal mouse anti-glucagon antibody were obtained from Sigma (St. Louis, MO). Total cholesterol, HDL-cholesterol, and triglyceride liquid reagents and their respective standards, fetal bovine serum, and penicillin-streptomycin were from Fisher Scientific (Pittsburgh, PA) and CMRL-1066 was from Invitrogen (Grand Island, NY). Insulin rabbit mAb and cleaved caspase 3 rabbit mAb were obtained from Cell Signaling (Danvers, MA). The secondary antibodies, DyLight 649-conjugated donkey anti-rabbit or anti-mouse IgG and Alexa 488-conjugated donkey anti-rabbit or anti-mouse antibody were obtained from Jackson ImmunoResearch Laboratories (West Grove, PA) and 4′-6-diamidino-2-phenylindole (DAPI) were obtained from Invitrogen (Grand Island, NY). Immunocruz rabbit ABC staining system was from Santa Cruz Biotechnology (Dallas, Texas) and 3-nitrotyrosine mouse mAb was from Abcam (Cambridge, MA). Insulin mouse mAb and rat insulin RIA kit was from Millipore (St. Louis, MO). All other chemicals were from commercially available sources.
Johns M., Fyalka R., Shea J.A., Neumann W.L., Rausaria S., Msengi E.N., Imani-Nejad M., Zollars H., McPherson T., Schober J., Wooten J, & Kwon G. (2015). SR-135, a Peroxynitrite Decomposing Catalyst, Enhances β-cell Function and Survival in B6D2F1 Mice Fed a High Fat Diet. Archives of biochemistry and biophysics, 0, 49-59.
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9

Rodent Diet and Biomarker Analysis

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Laboratory Rodent Diet 5001 was purchased from EL Mel (St. Louis, MO) and Sucrose Dustless Precision Pellets were from Bio-Serve (Flemington, NJ). Insulin rabbit mAb was obtained from Cell Signaling (Danvers, MA). Stretozotocin and monoclonal mouse anti-glucagon antibody were obtained from Sigma (St. Louis, MO). The secondary antibodies, Alexa 647-conjugated donkey anti-rabbit antibodies and Alexa 488-conjugated donkey anti-mouse were obtained from Jackson Immuno Research Laboratories (West Grove, PA). Schiff reagent, periodic acid, total cholesterol, high density lipoprotein (HDL)-cholesterol, and triacylglyceride (TAG) liquid reagents and their respective standards were purchased from Fisher Scientific (Pittsburgh, PA) and alpha-amylase was from MilliporeSigma (Burlington, MA). Contour Next test strips were obtained from ADW Diabetes (Pompano Beach, FL). Rat hemoglobin A1c kit and control for the kit were obtained from Crystal Chem (Elk Grove Village, IL).
Kirilmaz O.B., Salegaonkar A.R., Shiau J., Uzun G., Ko H.S., Lee H.F., Park S, & Kwon G. (2021). Study of blood glucose and insulin infusion rate in real-time in diabetic rats using an artificial pancreas system. PLoS ONE, 16(7), e0254718.
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