6-gingerol and propidium iodide (PI) were purchased from Sigma Aldrich (St. Louis, CA, USA). Briefly, 6-gingerol was dissolved in DMSO as a 34 mM stock solution and stored at -20 °C. 3-(4-dimethylthiazol-2-yl)-2-5-diphenyl tetrazolium bromide (MTT) was purchased from Invitrogen (Walthem, MA, USA). Annexin V assay kit was purchased from BD Biosciences (Palo Alto, CA, USA). Ultra-pure water was used for the preparation of reagents as well as in all experiments.
3 4 dimethylthiazol 2 yl 2 5 diphenyl tetrazolium bromide mtt
Manufactured by Thermo Fisher Scientific
Sourced in United States
3-(4-dimethylthiazol-2-yl)-2-5-diphenyl tetrazolium bromide (MTT) is a colorimetric assay used to measure cellular metabolic activity. It is a pale yellow tetrazolium salt that is reduced by metabolically active cells to a purple formazan product, which can be quantified spectrophotometrically.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 3 4 dimethylthiazol 2 yl 2 5 diphenyl tetrazolium bromide mtt
1
Induction of Apoptosis by 6-Gingerol
2
Menthol-Induced Apoptosis and Autophagy
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Menthol (Sigma-Aldrich, Schnelldorf, Germany, Cat No. 63660) was purchased from Sigma Aldrich (St. Louis, CA, USA) and dissolved in DMSO for storage at 4 °C. Moreover, RNase A and propidium iodide (PI) were purchased from Sigma Aldrich (St. Louis, CA, USA). 3-(4-dimethylthiazol-2-yl)-2-5-diphenyl tetrazolium bromide (MTT) and Annexin V-FITC apoptosis assay kit was obtained from Invitrogen (Walthem, MA, USA) and BD Bioscience (Palo Alto, CA, USA), respectively. Autophagy LC3-II antibody-based assay kit was purchased from Luminex Corporation (Austin, TX, USA). All other reagents used in the experiments were commercially analytical grade and available.
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