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Enzymatic colorimetric kit

Manufactured by Merck Group
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Sourced in Germany, France

Enzymatic colorimetric kits are laboratory equipment used to measure the concentration of specific analytes in a sample through a colorimetric reaction. The kits contain all the necessary reagents and components to perform the analysis. The core function of these kits is to provide a standardized and reliable method for quantitative analysis of target molecules in various biological, clinical, or research applications.

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Lab products found in correlation

Bead ruptor24e, by Omni International (2 mentions) Insulin, by Mercodia (1 mentions) Accu chek glucometer, by Roche (1 mentions) Enzyme linked immunosorbent assay kit, by R&D Systems (1 mentions) Leptin, by R&D Systems (1 mentions) Ultrasensitive mouse elisa kit, by Mercodia (1 mentions)

Close spelling variants of this product

Enzymatic kit

4 protocols using enzymatic colorimetric kit

1

Metabolic Biomarker Quantification

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An Accu-Chek Glucometer (Roche Diagnostics, Barcelona, Spain) was used to measure blood glucose concentration. Enzyme-linked immunosorbent assay kits were used for the quantification of leptin (R&D Systems, Minneapolis, MN, USA) and insulin (Mercodia AB, Uppsala, Sweden) in plasma samples. Enzymatic colorimetric kits were used for the quantification of plasma triglyceride (TG) levels (Sigma) and nonesterified (or free) fatty acids (Wako Chemicals GmbH, Neuss, Germany).
Pomar C.A., Picó C., Palou A, & Sánchez J. (2022). Maternal Consumption of a Cafeteria Diet during Lactation Leads to Altered Diet-Induced Thermogenesis in Descendants after Exposure to a Western Diet in Adulthood. Nutrients, 14(9), 1958.
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2

Comprehensive Lipid and Metabolite Analysis

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Circulating levels of HDL-cholesterol, LDL-cholesterol, triglycerides, free fatty acids, ALP, ALT, and fructosamine were determined with an autoanalyzer (Cobas Roche Diagnostic, Basel, Switzerland). Insulin was assayed with an ultrasensitive mouse ELISA kit (Mercodia, Uppsala, Sweden). Serum glycerol and hepatic triacylglycerol were determined by using enzymatic colorimetric kits (Sigma–Aldrich, Saint-Quentin-Fallavier, France). Organ lipid content was determined according to Dole and Meinertz [39 (link)]. Protein content was determined with the BCA protein assay kit (Pierce, Rockford, IL, USA). DNA content was determined with a fluorometric method.
Carpéné C., Gómez-Zorita S., Chaplin A, & Mercader J. (2018). Metabolic Effects of Oral Phenelzine Treatment on High-Sucrose-Drinking Mice. International Journal of Molecular Sciences, 19(10), 2904.
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3

Glycogen Content Quantification in Liver

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Liver pieces were weighted, and homogenized in water using Bead Ruptor24e (OMNI) with stainless steel beads. The extracts were boiled for 10 min, and then were centrifuged for 15 min in 21,000 x g (4°C). Next, glycogen content was quantified using an enzymatic colorimetric kit (Sigma), with equal tissue concentration (30 ug / well). The background glucose measurements (i.e., taken without hydrolysis of the glycogen) were used for background subtraction, as well as for measuring the tissue glucose content.
Manella G., Sabath E., Aviram R., Dandavate V., Ezagouri S., Golik M., Adamovich Y, & Asher G. (2021). The liver-clock coordinates rhythmicity of peripheral tissues in response to feeding. Nature metabolism, 3(6), 829-842.
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4

Glycogen Content Quantification in Liver

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Liver pieces were weighted, and homogenized in water using Bead Ruptor24e (OMNI) with stainless steel beads. The extracts were boiled for 10 min, and then were centrifuged for 15 min in 21,000 x g (4°C). Next, glycogen content was quantified using an enzymatic colorimetric kit (Sigma), with equal tissue concentration (30 ug / well). The background glucose measurements (i.e., taken without hydrolysis of the glycogen) were used for background subtraction, as well as for measuring the tissue glucose content.
Manella G., Sabath E., Aviram R., Dandavate V., Ezagouri S., Golik M., Adamovich Y, & Asher G. (2021). The liver-clock coordinates rhythmicity of peripheral tissues in response to feeding. Nature metabolism, 3(6), 829-842.
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