The tissue section was prepared as in IHC method. Primary antibodies include anti-GBP5 (Cat. #: 67798, Cell Signaling, United States), anti-CD3 (Cat. #: 60181-1-Ig, Proteintech, China), anti-CD40 (Cat. #: ab280207, Abcam, United States), and anti-CD68 (Cat. #: ARG10514, Arigo, China). Secondary antibodies include Alexa Fluor647 or 488 conjugated goat anti-rabbit or goat anti-mouse antibodies (Invitrogen, United States). Cell nuclei were stained with 4′,6-diamidine-2′-phenylindole dihydrochloride (DAPI, Sigma-Aldrich, United States). Slides were visualized with a TCS-SP8 confocal microscope (Leica, Germany).
Anti cd3
Manufactured by Proteintech
Sourced in United States, China
Anti-CD3 is a laboratory reagent used in immunological research. It binds to the CD3 complex, a key component of the T-cell receptor. This interaction can be used to activate and study T-cell function in various experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
4 6 diamidine 2 phenylindole dihydrochloride dapi, by Merck Group (1 mentions)
Anti gbp5, by Cell Signaling Technology (1 mentions)
Tcs sp8 confocal microscope, by Leica (1 mentions)
Anti cd40, by Proteintech (1 mentions)
Anti cd68, by Arigo Biolaboratories (1 mentions)
Anti foxp3, by Cell Signaling Technology (1 mentions)
Anti p akt, by Proteintech (1 mentions)
Anti akt, by Proteintech (1 mentions)
Image pro plus 6.0 image analysis system, by Media Cybernetics (1 mentions)
Optical microscope, by Olympus (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions)
Anti ran, by Proteintech (1 mentions)
3 protocols using anti cd3
1
Immunofluorescence Analysis of Immune Markers
2
Immunohistochemical Analysis of Colonic Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The tissue samples were stained according to the manufacturer's protocol using an immunohistochemistry kit, and the immunohistochemical images were analyzed and counted by the image pro-Plus 6.0 image analysis system (Media Cybernetics, Inc., Rockville, MD, USA), and the positive expression area and positive expression integral optical density (IOD) was measured. Immunohistochemistry (IHC) was performed on paraffin-embedded colonic tissue sections complying with a standard procedure. 33 Slices were then incubated with specific primary antibodies for anti-AKT, anti-p-AKT, anti-CD3 (1:200, 1:200, 1:500; Proteintech Group, Inc., Wuhan, China) and anti-Foxp3 (1:200; Cell Signaling Technology, Danvers, MA, USA) overnight at 4 , followed by a biotinylated secondary antibody. Sections were developed with diaminobenzidine (DAB) and counterstained with hematoxylin. Images of IHC slides were visualized and analyzed using image pro-Plus 6.0 image analysis system.
3
Immunohistochemical and Immunofluorescence Analysis of DLBCL
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunohistochemistry, as well as immunofluorescence on lymph node biopsies from DLBCL patients, were conducted as outlined previously [26 (link)]. The sections were stained using a primary antibody (anti-MECR, Proteintech, Cat. NO: 51027-2-AP; anti-RAN, Proteintech, Cat. NO: 67500-1-Ig; anti-ARSK, Bioss, Cat. NO: bs-9102R; anti-CD3, Proteintech, Cat. NO: 60181-1-Ig; anti-CD20, Proteintech, Cat. NO: 60271-1-Ig). The nucleus was stained using DAPI (Solarbio, Beijing, China) for use in immunofluorescence. Images of stained slides for these markers were scanned at 400× magnification using an optical microscope (Olympus Co., Tokyo, Japan). Immunohistochemistry results were quantified by counting the area of positive signals using Image J software. Fluorescent images were captured via a confocal laser microscopy system (Leica SP2).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!