Edta disodium salt titriplex 3 citric acid

Analytical grade chemicals were used in sample and mobile-phase preparations. Perchloric acid (HClO4), Na2S2)2, EDTA-disodium salt (titriplex III) citric acid, orthophosphoric acid, hydrochloric acid, sodium chloride, methanol (Lichorov), Na2HPO4, and 2-mercaptoethanol were purchased from Merck (Darmstadt). Dihydroxybenzilamine (DHBA), 6-hidroxydopamine, pilocarpine hydrochloride, and scopolamine methylnitrate were purchased from Sigma (St. Louis, MO, USA). Dopamine depletion in epilepic rats of this model, meaning that animals became epileptic. After that, epileptic animals were randomly assigned to two groups: 6-OHDA: 16 epileptic rats, received 6-OHDA into the substantia nigra stereotaxically; and, Control: 11 epileptic rats, received ascorbic acid in the same nucleus. Aiming to avoid inflammatory interference in the occurrence of seizure frequency, animals were let to rest for 2 weeks, when they returned to the video-monitoring room for additional 3 weeks. In this period, the frequency of SRSs was recorded. After this period, the seizure frequency of each animal was compared with that observed before receiving 6-OHDA treatment (Figure 1).

Analytical grade chemicals were used in sample and mobile-phase preparations. Perchloric acid (HClO4), Na2S2)2, EDTA-disodium salt (titriplex III) citric acid, orthophosphoric acid, hydrochloric acid, sodium chloride, methanol (Lichorov), Na2HPO4, and 2-mercaptoethanol were purchased from Merck (Darmstadt). Dihydroxybenzilamine (DHBA), 6-hidroxydopamine, pilocarpine hydrochloride, and scopolamine methylnitrate were purchased from Sigma (St. Louis, MO, USA). Dopamine depletion in epilepic rats of this model, meaning that animals became epileptic. After that, epileptic animals were randomly assigned to two groups: 6-OHDA: 16 epileptic rats, received 6-OHDA into the substantia nigra stereotaxically; and, Control: 11 epileptic rats, received ascorbic acid in the same nucleus. Aiming to avoid inflammatory interference in the occurrence of seizure frequency, animals were let to rest for 2 weeks, when they returned to the video-monitoring room for additional 3 weeks. In this period, the frequency of SRSs was recorded. After this period, the seizure frequency of each animal was compared with that observed before receiving 6-OHDA treatment (Figure 1).