All reagents used in this section were from Thermo Fisher Scientific (Waltham, MA, USA) unless specified.
Twelve-well Novex® 4–20% Tris-Glycine Mini Protein Gels (Thermo Fisher Scientific) were run at 150 V for 90 min with Novex® Native Tris-Glycine Running Buffer System at room temperature. Novex® Unstained Protein Standard and Novex® Native 2X Sample Buffer were used in the electrophoresis. The gels were stained by Coomassie Blue Staining (GE Healthcare Bio-Science AB, Uppsala, Sweden) for visualization or Imperial™ Protein Stain for visualization and further mass spectrometric analysis. The gel image was captured using a Bio-Rad GS-800 calibrated densitometer (Bio- Rad Laboratories, Hercules, CA, USA).
Twelve-well Novex® 4–20% Tris-Glycine Mini Protein Gels (Thermo Fisher Scientific) were run at 150 V for 90 min with Novex® Native Tris-Glycine Running Buffer System at room temperature. Novex® Unstained Protein Standard and Novex® Native 2X Sample Buffer were used in the electrophoresis. The gels were stained by Coomassie Blue Staining (GE Healthcare Bio-Science AB, Uppsala, Sweden) for visualization or Imperial™ Protein Stain for visualization and further mass spectrometric analysis. The gel image was captured using a Bio-Rad GS-800 calibrated densitometer (Bio- Rad Laboratories, Hercules, CA, USA).