Plasma was separated from EDTA whole blood by centrifugation at 2000 G for 15 min at 4°C. A 1:50 dilution was prepared using SDS-PAGE loading buffer. Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes for immunoblotting. Membranes were probed with polyclonal primary Abs: rabbit anti-CSF1 (1:500, MBS551037; MyBioSource) and 1:2000 sheep anti-Transferrin (1:2000, ab9033; Abcam) in PBS/3% milk powder/0.1% (v/v) Tween 20 at 4°C overnight. The secondary Abs used were goat anti-rabbit HRP (1:5000, no. 7074; Cell Signaling Technologies) and rabbit anti-sheep HRP (1:10,000, ab97130; Abcam). Blots were visualized using Pierce ECL Western blotting solution (Thermo Fisher Scientific).
Ab9033
Manufactured by Abcam
Sourced in United Kingdom
Ab9033 is a polyclonal antibody that targets the protein Amyloid Precursor Protein (APP). This antibody is suitable for use in various applications, including Western blotting, immunohistochemistry, and immunocytochemistry.
Automatically generated - may contain errors
2 protocols using ab9033
1
Quantification of CSF1 and Transferrin in Plasma
2
Transferrin Immunofluorescence Staining
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293 cells were washed with phosphate-buffered saline (PBS), fixed using 4% paraformaldehyde for 15 min at room temperature, and washed again with PBS. Cells were permeabilized using 0.2% Triton X-100 in PBS for 15 min at room temperature, followed by washing the samples with PBS. Samples were incubated for 1 hour at room temperature in blocking buffer (PBS with 10% bovine serum albumin). Anti-transferrin antibody (Abcam ab9033, England) was used as primary antibody and incubated in blocking for 2 hours at room temperature; after washing with PBS cells were incubated for 45 min at room temperature with an anti-rabbit secondary antibody diluted in blocking buffer [21] .
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